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Yorodumi- PDB-2ej7: Solution structure of the DnaJ domain of the human protein HCG3, ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2ej7 | ||||||
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Title | Solution structure of the DnaJ domain of the human protein HCG3, a hypothetical protein tmp_locus_21 | ||||||
Components | HCG3 gene | ||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / HCG3 protein / DnaJ domain / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information chaperone-mediated protein folding / Hsp70 protein binding / unfolded protein binding Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Ohinishi, S. / Tochio, N. / Koshiba, S. / Inoue, M. / Kigawa, T. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: To be Published Title: Solution structure of the DnaJ domain of the human protein HCG3, a hypothetical protein tmp_locus_21 Authors: Ohnishi, S. / Tochio, N. / Koshiba, S. / Inoue, M. / Kigawa, T. / Yokoyama, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2ej7.cif.gz | 492.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2ej7.ent.gz | 414.8 KB | Display | PDB format |
PDBx/mmJSON format | 2ej7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2ej7_validation.pdf.gz | 342.9 KB | Display | wwPDB validaton report |
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Full document | 2ej7_full_validation.pdf.gz | 461.3 KB | Display | |
Data in XML | 2ej7_validation.xml.gz | 30.9 KB | Display | |
Data in CIF | 2ej7_validation.cif.gz | 47.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ej/2ej7 ftp://data.pdbj.org/pub/pdb/validation_reports/ej/2ej7 | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 9258.234 Da / Num. of mol.: 1 / Fragment: DnaJ domain, residues 8-76 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Description: cell-free protein synthesis / Gene: HCG3 / Plasmid: P050704-06 / References: UniProt: Q8WWF6 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.0mM sample U-15N,13C; 20mM d-Tris-HCl; 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 120mM / pH: 7 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 800 MHz |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations, target function Conformers calculated total number: 100 / Conformers submitted total number: 20 |