+
Open data
-
Basic information
Entry | Database: PDB / ID: 2eip | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | INORGANIC PYROPHOSPHATASE | |||||||||
![]() | SOLUBLE INORGANIC PYROPHOSPHATASE | |||||||||
![]() | INORGANIC PYROPHOSPHATASE / HYDROLASE / MAGNESIUM / INORGANIC PYROPHOSHATASE | |||||||||
Function / homology | ![]() inorganic triphosphate phosphatase activity / inorganic diphosphatase / inorganic diphosphate phosphatase activity / phosphate-containing compound metabolic process / magnesium ion binding / zinc ion binding / membrane / cytosol Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() | |||||||||
![]() | Kankare, J.A. / Salminen, T. / Goldman, A. | |||||||||
![]() | ![]() Title: Structure of Escherichia coli inorganic pyrophosphatase at 2.2 A resolution. Authors: Kankare, J. / Salminen, T. / Lahti, R. / Cooperman, B.S. / Baykov, A.A. / Goldman, A. #1: ![]() Title: New Crystal Forms of Escherichia Coli and Saccharomyces Cerevisiae Soluble Inorganic Pyrophosphatase Authors: Heikinheimo, P. / Salminen, T. / Cooperman, B. / Lahti, R. / Goldman, A. #2: ![]() Title: The Structure of E.Coli Soluble Inorganic Pyrophosphatase at 2.7 A Resolution Authors: Kankare, J. / Neal, G.S. / Salminen, T. / Glumhoff, T. / Cooperman, B.S. / Lahti, R. / Goldman, A. | |||||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 79.6 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 61.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 421.6 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 423.4 KB | Display | |
Data in XML | ![]() | 15.4 KB | Display | |
Data in CIF | ![]() | 21.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
Unit cell |
| ||||||||
Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.51, 0.86, 0.005), Vector: Details | THE ACTIVE ENZYME IS A HEXAMER. THE ASYMMETRIC UNIT OF THIS CRYSTAL FORM CONTAINS TWO MONOMERS OF THE ACTIVE HEXAMER. THE WHOLE HEXAMER CAN BE GENERATED BY APPLYING TWICE THE CRYSTALLOGRAPHIC THREE-FOLD ROTATIONS AROUND C-AXIS. THE TWO MONOMERS IN THE ASYMMETRIC UNIT ARE RELATED BY A LOCAL TWO-FOLD OPERATOR PARALLEL TO THE CRYSTALLOGRAPHIC TWO-FOLD. | |
-
Components
#1: Protein | Mass: 19597.334 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #2: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 47.07 % | ||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | *PLUS pH: 8 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction source | Wavelength: 1.5418 |
---|---|
Detector | Type: RIGAKU / Detector: IMAGE PLATE |
Radiation | Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→20 Å / Num. obs: 16178 / % possible obs: 86.5 % / Observed criterion σ(I): 2 / Rmerge(I) obs: 0.082 |
Reflection | *PLUS Num. measured all: 140425 |
Reflection shell | *PLUS Highest resolution: 2.2 Å / Lowest resolution: 2.3 Å / % possible obs: 72 % / Mean I/σ(I) obs: 3 |
-
Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 2.2→8 Å / σ(F): 2
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 30.5 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→8 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
|