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Yorodumi- PDB-1ipw: INORGANIC PYROPHOSPHATASE FROM ESCHERICHIA COLI WITH THREE MAGNES... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1ipw | ||||||
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Title | INORGANIC PYROPHOSPHATASE FROM ESCHERICHIA COLI WITH THREE MAGNESIUM IONS | ||||||
Components | SOLUBLE INORGANIC PYROPHOSPHATASE | ||||||
Keywords | HYDROLASE / SOLUBLE INORGANIC PYROPHOSPHATASE / MAGNESIUM | ||||||
Function / homology | Function and homology information inorganic triphosphate phosphatase activity / inorganic diphosphatase / inorganic diphosphate phosphatase activity / phosphate-containing compound metabolic process / magnesium ion binding / zinc ion binding / membrane / cytosol Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.3 Å | ||||||
Authors | Kankare, J.A. / Goldman, A. | ||||||
Citation | Journal: Biochemistry / Year: 1996 Title: Crystallographic identification of metal-binding sites in Escherichia coli inorganic pyrophosphatase. Authors: Kankare, J. / Salminen, T. / Lahti, R. / Cooperman, B.S. / Baykov, A.A. / Goldman, A. #1: Journal: To be Published Title: Structure of Escherichia Coli Inorganic Pyrophosphatase at 2.2 Angstroms Resolution Authors: Kankare, J. / Salminen, T. / Lahti, R. / Cooperman, B.S. / Baykov, A.A. / Goldman, A. #2: Journal: Acta Crystallogr.,Sect.D / Year: 1995 Title: New Crystal Forms of Escherichia Coli and Saccharomyces Cerevisiae Soluble Inorganic Pyrophosphatase Authors: Heikinheimo, P. / Salminen, T. / Cooperman, B. / Lahti, R. / Goldman, A. #3: Journal: Protein Eng. / Year: 1994 Title: The Structure of E.Coli Soluble Inorganic Pyrophosphatase at 2.7 A Resolution Authors: Kankare, J. / Neal, G.S. / Salminen, T. / Glumoff, T. / Cooperman, B.S. / Lahti, R. / Goldman, A. #4: Journal: Protein Eng. / Year: 1994 Title: Erratum. The Structure of E.Coli Soluble Inorganic Pyrophosphatase at 2.7 A Resolution Authors: Kankare, J. / Neal, G.S. / Salminen, T. / Glumoff, T. / Cooperman, B.S. / Lahti, R. / Goldman, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ipw.cif.gz | 80.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ipw.ent.gz | 61.4 KB | Display | PDB format |
PDBx/mmJSON format | 1ipw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ip/1ipw ftp://data.pdbj.org/pub/pdb/validation_reports/ip/1ipw | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.504066, 0.863638, 0.006824), Vector: |
-Components
#1: Protein | Mass: 19597.334 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Escherichia coli (E. coli) / References: UniProt: P0A7A9, inorganic diphosphatase #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.27 Å3/Da / Density % sol: 45.7 % | |||||||||||||||
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Crystal grow | *PLUS pH: 8 / Method: other | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: SEALED TUBE / Wavelength: 1.5418 |
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Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE |
Radiation | Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 13745 / % possible obs: 79.7 % / Observed criterion σ(I): 2 / Rmerge(I) obs: 0.048 |
Reflection | *PLUS Highest resolution: 2.3 Å |
Reflection shell | *PLUS Highest resolution: 2.3 Å / Lowest resolution: 2.4 Å / % possible obs: 67.9 % |
-Processing
Software |
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Refinement | Resolution: 2.3→8 Å / σ(F): 2
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Displacement parameters | Biso mean: 24.4 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→8 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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