[English] 日本語
![](img/lk-miru.gif)
- PDB-2egu: Crystal structure of O-acetylserine sulfhydrase from Geobacillus ... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 2egu | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal structure of O-acetylserine sulfhydrase from Geobacillus kaustophilus HTA426 | ||||||
![]() | Cysteine synthase | ||||||
![]() | TRANSFERASE / O-acetylserine sulfhydrase / Structural Genomics / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | ![]() cysteine synthase / cysteine synthase activity / cysteine biosynthetic process from serine Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Rehse, P.H. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
![]() | ![]() Title: Crystal structure of O-acetylserine sulfhydrase from Geobacillus kaustophilus HTA426 Authors: Rehse, P.H. / Yokoyama, S. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 68.9 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 49.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | ![]() 1z7wS S: Starting model for refinement |
---|---|
Similar structure data | |
Other databases |
-
Links
-
Assembly
Deposited unit | ![]()
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
2 | ![]()
| |||||||||
3 | ![]()
| |||||||||
Unit cell |
| |||||||||
Components on special symmetry positions |
|
-
Components
#1: Protein | Mass: 32555.275 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
---|---|
#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.55 Å3/Da / Density % sol: 51.68 % |
---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.3 Details: 100mM MES-NaOH, 50mM MgCl2, 27.5% PEG 4000, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RIGAKU RAXIS V / Detector: IMAGE PLATE / Date: Dec 16, 2006 / Details: mirrors |
Radiation | Monochromator: Si (111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9794 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→40 Å / Num. all: 27260 / Num. obs: 27236 / % possible obs: 99.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 14.3 % / Rmerge(I) obs: 0.107 / Net I/σ(I): 36.86 |
Reflection shell | Resolution: 1.9→1.97 Å / Redundancy: 14.6 % / Rmerge(I) obs: 0.529 / Mean I/σ(I) obs: 5.22 / Num. unique all: 2654 / % possible all: 100 |
-
Processing
Software |
| ||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: 1Z7W.pdb Resolution: 1.9→39.81 Å / Isotropic thermal model: anisotropic / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
| ||||||||||||||||||||||||||||
Solvent computation | Bsol: 35.998 Å2 | ||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 28.574 Å2
| ||||||||||||||||||||||||||||
Refine analyze |
| ||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→39.81 Å
| ||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||
LS refinement shell | Resolution: 1.9→1.97 Å
| ||||||||||||||||||||||||||||
Xplor file |
|