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- PDB-2e2a: ASP81LEU ENZYME IIA FROM THE LACTOSE SPECIFIC PTS FROM LACTOCOCCU... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2e2a | ||||||
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Title | ASP81LEU ENZYME IIA FROM THE LACTOSE SPECIFIC PTS FROM LACTOCOCCUS LACTIS | ||||||
![]() | PROTEIN (ENZYME IIA) | ||||||
![]() | TRANSFERASE / ENZYME IIA / HELICAL BUNDLES / PTS / PHOSPHOTRANSFERASE SYSTEM | ||||||
Function / homology | ![]() phosphoenolpyruvate-dependent sugar phosphotransferase system / transferase activity / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Sliz, P. / Koch, B. / Hengstenberg, W. / Pai, E.F. | ||||||
![]() | ![]() Title: Structure of Asp81Leu Enzyme Iia from the Lactose Specific Pts from Lactococcus Lactis Authors: Sliz, P. / KOCH, B. / Hengstenberg, W. / Pai, E.F. #1: ![]() Title: The Structure of Enzyme Iialactose from Lactococcus Lactis Reveals a New Fold and Points to Possible Interactions of a Multicomponent System Authors: Sliz, P. / Engelmann, R. / Hengstenberg, W. / Pai, E.F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 71.7 KB | Display | ![]() |
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PDB format | ![]() | 54.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 444.8 KB | Display | ![]() |
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Full document | ![]() | 450.3 KB | Display | |
Data in XML | ![]() | 15.2 KB | Display | |
Data in CIF | ![]() | 21.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1e2aS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 11461.188 Da / Num. of mol.: 3 / Mutation: D81L Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: MG1820 / Description: NIZO, NETHERLANDS / Gene: LACF / Production host: ![]() ![]() References: UniProt: P23532, protein-Npi-phosphohistidine-sugar phosphotransferase #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.25 Å3/Da / Density % sol: 44.56 % |
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Crystal grow | pH: 6.5 Details: THE PROTEIN WAS CRYSTALLIZED FROM 0.9M NA ACETATE AND 0.1M NA CACODYLATE AT PH 6.5. HANGING-DROP METHOD WAS USED. THE INITIAL CONCENTRATION OF THE PROTEIN IN THE DROP WAS 5 MG/ML. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Mar 30, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.949 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→36 Å / Num. obs: 18726 / % possible obs: 99.5 % / Observed criterion σ(I): 0 / Redundancy: 4.8 % / Biso Wilson estimate: 30.6 Å2 / Rsym value: 0.037 / Net I/σ(I): 13.7 |
Reflection shell | Resolution: 2.1→2.2 Å / Redundancy: 4.2 % / Mean I/σ(I) obs: 2.7 / Rsym value: 0.271 / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure: OTHER Starting model: 1E2A Resolution: 2.1→20 Å / Rfactor Rfree error: 0.006 / Data cutoff high rms absF: 1666967.76 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 59.47 Å2 / ksol: 0.3684 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 45.9 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.1→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.1→2.23 Å / Rfactor Rfree error: 0.017 / Total num. of bins used: 6
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Xplor file |
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