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Open data
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Basic information
Entry | Database: PDB / ID: 2dyn | ||||||
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Title | DYNAMIN (PLECKSTRIN HOMOLOGY DOMAIN) (DYNPH) | ||||||
![]() | DYNAMIN | ||||||
![]() | SIGNAL TRANSDUCTION / MOTOR PROTEIN / PHOSPHOLIPID BINDING / PROTEIN BINDING | ||||||
Function / homology | ![]() clathrin coat assembly involved in endocytosis / vesicle scission / synaptic vesicle budding from presynaptic endocytic zone membrane / presynaptic endocytic zone membrane / dynamin GTPase / chromaffin granule / regulation of vesicle size / Toll Like Receptor 4 (TLR4) Cascade / Retrograde neurotrophin signalling / Formation of annular gap junctions ...clathrin coat assembly involved in endocytosis / vesicle scission / synaptic vesicle budding from presynaptic endocytic zone membrane / presynaptic endocytic zone membrane / dynamin GTPase / chromaffin granule / regulation of vesicle size / Toll Like Receptor 4 (TLR4) Cascade / Retrograde neurotrophin signalling / Formation of annular gap junctions / endosome organization / Gap junction degradation / photoreceptor ribbon synapse / membrane coat / Recycling pathway of L1 / phosphatidylinositol-3,4,5-trisphosphate binding / endocytic vesicle / EPH-ephrin mediated repulsion of cells / clathrin-coated pit / phosphatidylinositol-4,5-bisphosphate binding / MHC class II antigen presentation / photoreceptor inner segment / receptor-mediated endocytosis / cell projection / modulation of chemical synaptic transmission / protein homooligomerization / receptor internalization / endocytosis / GDP binding / : / presynapse / Clathrin-mediated endocytosis / microtubule binding / protein homotetramerization / microtubule / GTPase activity / glutamatergic synapse / synapse / GTP binding / protein kinase binding / protein homodimerization activity / RNA binding / extracellular exosome / identical protein binding / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Timm, D.E. | ||||||
![]() | ![]() Title: Crystal structure of the pleckstrin homology domain from dynamin. Authors: Timm, D. / Salim, K. / Gout, I. / Guruprasad, L. / Waterfield, M. / Blundell, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 58.1 KB | Display | ![]() |
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PDB format | ![]() | 43.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 437.6 KB | Display | ![]() |
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Full document | ![]() | 440.8 KB | Display | |
Data in XML | ![]() | 10.9 KB | Display | |
Data in CIF | ![]() | 14.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.847437, -0.116183, -0.518027), Vector: |
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Components
#1: Protein | Mass: 14430.390 Da / Num. of mol.: 2 / Fragment: PLECKSTRIN HOMOLOGY (PH) DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Description: EXPRESSED AS GST-FUSION PROTEIN, CLEAVED WITH THROMBIN Plasmid: PGEX-2T / Production host: ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.21 Å3/Da / Density % sol: 44.3 % | |||||||||||||||
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Crystal grow | pH: 6.1 Details: PROTEIN (10-20 MG/ML) WAS CRYSTALLIZED FROM 10-30% PEG 8000, 0.2 M AMMONIUM SULFATE, PH 6.1 BY THE HANGING DROP VAPOR DIFFUSION METHOD | |||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jul 1, 1995 / Details: SYNCHROTRON |
Radiation | Monochromator: SYNCHROTRON / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.87 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→24.31 Å / Num. obs: 11759 / % possible obs: 99.5 % / Observed criterion σ(I): 2 / Redundancy: 6.6 % / Rmerge(I) obs: 0.058 / Rsym value: 0.058 / Net I/σ(I): 6.5 |
Reflection shell | Resolution: 2.3→2.42 Å / Redundancy: 6.3 % / Rmerge(I) obs: 0.144 / Mean I/σ(I) obs: 5.1 / Rsym value: 0.144 / % possible all: 99.3 |
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Processing
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Refinement | Method to determine structure: MIR WITH ANOMALOUS SCATTERING Resolution: 2.3→24.31 Å / Data cutoff high absF: 100000 / Data cutoff low absF: 0.1 / Cross valid method: POSTERIORI / σ(F): 2 Details: A BULK SOLVENT MASK WAS APPLIED IN XPLOR 3.1. RESIDUES WITH OCCUPANCY OF 0.00 ARE INCLUDED IN THE MODEL, BUT ARE NOT DEFINED BY THE ELECTRON DENSITY.
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Displacement parameters | Biso mean: 38.5 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→24.31 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.3→2.4 Å / Total num. of bins used: 8
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Xplor file |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor Rfree: 0.2966 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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