- PDB-2doq: crystal structure of Sfi1p/Cdc31p complex -
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基本情報
登録情報
データベース: PDB / ID: 2doq
タイトル
crystal structure of Sfi1p/Cdc31p complex
要素
Cell division control protein 31
SFI1p
キーワード
CELL CYCLE / Sfi1p / centrin / cdc31p / spindle pole body / centrosome
機能・相同性
機能・相同性情報
half bridge of spindle pole body / spindle pole body duplication / mitotic spindle pole body / Golgi vesicle transport / transcription export complex 2 / spindle assembly / mRNA export from nucleus / spindle pole / G2/M transition of mitotic cell cycle / nuclear envelope ...half bridge of spindle pole body / spindle pole body duplication / mitotic spindle pole body / Golgi vesicle transport / transcription export complex 2 / spindle assembly / mRNA export from nucleus / spindle pole / G2/M transition of mitotic cell cycle / nuclear envelope / microtubule binding / proteasome-mediated ubiquitin-dependent protein catabolic process / cell division / calcium ion binding / positive regulation of transcription by RNA polymerase II / identical protein binding / cytoplasm 類似検索 - 分子機能
Single alpha-helices involved in coiled-coils or other helix-helix interfaces - #1000 / Sfi1 spindle body / Spindle body associated protein, C-terminal domain / Sfi1 spindle body protein / Spindle body associated protein C-terminus / Single alpha-helices involved in coiled-coils or other helix-helix interfaces / EF-hand / Recoverin; domain 1 / EF-hand domain pair / Helix non-globular ...Single alpha-helices involved in coiled-coils or other helix-helix interfaces - #1000 / Sfi1 spindle body / Spindle body associated protein, C-terminal domain / Sfi1 spindle body protein / Spindle body associated protein C-terminus / Single alpha-helices involved in coiled-coils or other helix-helix interfaces / EF-hand / Recoverin; domain 1 / EF-hand domain pair / Helix non-globular / EF-hand, calcium binding motif / Special / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / Orthogonal Bundle / Mainly Alpha 類似検索 - ドメイン・相同性
Cell division control protein 31 / Protein SFI1 類似検索 - 構成要素
解像度: 3.25→3.43 Å / 冗長度: 6.8 % / Rmerge(I) obs: 0.48 / Mean I/σ(I) obs: 1.3 / Rsym value: 0.013 / % possible all: 99.7
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解析
ソフトウェア
名称
バージョン
分類
REFMAC
5.2.0005
精密化
MOSFLM
データ削減
CCP4
(SCALA)
データスケーリング
SnB
位相決定
SHARP
位相決定
精密化
構造決定の手法: 多波長異常分散 / 解像度: 3→94.49 Å / Cor.coef. Fo:Fc: 0.924 / Cor.coef. Fo:Fc free: 0.904 / SU B: 48.46 / SU ML: 0.449 / 交差検証法: THROUGHOUT / σ(F): 1.1 / ESU R Free: 0.486 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.29899
785
5 %
RANDOM
Rwork
0.25857
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obs
0.26057
14942
99.49 %
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all
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14942
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK