[English] 日本語
Yorodumi- PDB-2dae: Solution Structure of the N-terminal CUE Domain in the Human Mito... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2dae | ||||||
---|---|---|---|---|---|---|---|
Title | Solution Structure of the N-terminal CUE Domain in the Human Mitogen-activated Protein Kinase Kinase Kinase 7 Interacting Protein 2 (MAP3K7IP2) | ||||||
Components | KIAA0733 protein | ||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / Mitogen-activated protein kinase kinase kinase 7 interacting protein 2 / MAP3K7IP2 / KIAA0733 / CUE domain / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information non-canonical NF-kappaB signal transduction / K63-linked polyubiquitin modification-dependent protein binding / positive regulation of protein kinase activity / Nuclear signaling by ERBB4 / IRAK2 mediated activation of TAK1 complex / Alpha-protein kinase 1 signaling pathway / IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation / TICAM1,TRAF6-dependent induction of TAK1 complex / TRAF6-mediated induction of TAK1 complex within TLR4 complex / negative regulation of autophagy ...non-canonical NF-kappaB signal transduction / K63-linked polyubiquitin modification-dependent protein binding / positive regulation of protein kinase activity / Nuclear signaling by ERBB4 / IRAK2 mediated activation of TAK1 complex / Alpha-protein kinase 1 signaling pathway / IRAK2 mediated activation of TAK1 complex upon TLR7/8 or 9 stimulation / TICAM1,TRAF6-dependent induction of TAK1 complex / TRAF6-mediated induction of TAK1 complex within TLR4 complex / negative regulation of autophagy / JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 / TNFR1-induced NF-kappa-B signaling pathway / ubiquitin binding / activated TAK1 mediates p38 MAPK activation / TAK1-dependent IKK and NF-kappa-B activation / NOD1/2 Signaling Pathway / CLEC7A (Dectin-1) signaling / FCERI mediated NF-kB activation / Interleukin-1 signaling / Downstream TCR signaling / heart development / positive regulation of canonical NF-kappaB signal transduction / response to lipopolysaccharide / molecular adaptor activity / endosome membrane / defense response to bacterium / inflammatory response / lysosomal membrane / SARS-CoV-2 activates/modulates innate and adaptive immune responses / zinc ion binding / nucleoplasm / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics, restrained molecular dynamics | ||||||
Authors | Zhao, C. / Kigawa, T. / Sato, M. / Koshiba, S. / Inoue, M. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: To be Published Title: Solution Structure of the N-terminal CUE Domain in the Human Mitogen-activated Protein Kinase Kinase Kinase 7 Interacting Protein 2 (MAP3K7IP2) Authors: Zhao, C. / Kigawa, T. / Sato, M. / Koshiba, S. / Inoue, M. / Yokoyama, S. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2dae.cif.gz | 421.6 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2dae.ent.gz | 352.7 KB | Display | PDB format |
PDBx/mmJSON format | 2dae.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2dae_validation.pdf.gz | 340.9 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 2dae_full_validation.pdf.gz | 479.4 KB | Display | |
Data in XML | 2dae_validation.xml.gz | 27.6 KB | Display | |
Data in CIF | 2dae_validation.cif.gz | 41.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/da/2dae ftp://data.pdbj.org/pub/pdb/validation_reports/da/2dae | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 8032.711 Da / Num. of mol.: 1 / Fragment: CUE domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Description: Cell-free protein synthesis / Gene: KIAA0733 / Plasmid: P050725-14 / References: GenBank: 20521143, UniProt: Q9NYJ8*PLUS |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details | Contents: 1.30mM CUE domain U-15N, 13C; 20mM d-Tris-HCl(pH 7.0); 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 90% H2O, 10%D2O Solvent system: 90% H2O/10% D2O |
---|---|
Sample conditions | Ionic strength: 120mM / pH: 7 / Pressure: ambient / Temperature: 296.0 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 700 MHz |
---|
-Processing
NMR software |
| ||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: torsion angle dynamics, restrained molecular dynamics Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function, structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 20 |