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Yorodumi- PDB-2d49: Solution structure of the Chitin-Binding Domain of Streptomyces g... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2d49 | ||||||
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Title | Solution structure of the Chitin-Binding Domain of Streptomyces griseus Chitinase C | ||||||
Components | chitinase C | ||||||
Keywords | HYDROLASE / chitinase / chitin binding domain / ChBD | ||||||
Function / homology | Function and homology information chitinase / chitinase activity / chitin catabolic process / cell wall macromolecule catabolic process / carbohydrate binding / carbohydrate metabolic process / extracellular region Similarity search - Function | ||||||
Biological species | Streptomyces griseus (bacteria) | ||||||
Method | SOLUTION NMR | ||||||
Authors | Akagi, K. / Watanabe, J. / Hara, M. / Kezuka, Y. / Chikaishi, E. / Yamaguchi, T. / Akutsu, H. / Nonaka, T. / Watanabe, T. / Ikegami, T. | ||||||
Citation | Journal: J.Biochem.(Tokyo) / Year: 2006 Title: Identification of the substrate interaction region of the chitin-binding domain of Streptomyces griseus chitinase C Authors: Akagi, K. / Watanabe, J. / Hara, M. / Kezuka, Y. / Chikaishi, E. / Yamaguchi, T. / Akutsu, H. / Nonaka, T. / Watanabe, T. / Ikegami, T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2d49.cif.gz | 492.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2d49.ent.gz | 430.6 KB | Display | PDB format |
PDBx/mmJSON format | 2d49.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2d49_validation.pdf.gz | 338 KB | Display | wwPDB validaton report |
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Full document | 2d49_full_validation.pdf.gz | 489.1 KB | Display | |
Data in XML | 2d49_validation.xml.gz | 20.2 KB | Display | |
Data in CIF | 2d49_validation.cif.gz | 34.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/d4/2d49 ftp://data.pdbj.org/pub/pdb/validation_reports/d4/2d49 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 5613.948 Da / Num. of mol.: 1 / Fragment: Chitin Binding Domain / Mutation: G82S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptomyces griseus (bacteria) / Plasmid: pET22b(+) / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: O50152, chitinase |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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-Sample preparation
Details | Contents: Napi 20mM DTT 1mM NaN, 0.02%(w/w); 90% H2O, 100% D2O Solvent system: 90% H2O, 100% D2O |
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Sample conditions | pH: 6 / Temperature: 303 K |
-NMR measurement
NMR spectrometer | Type: Bruker AV, DRX / Manufacturer: Bruker / Model: AV, DRX / Field strength: 400 MHz |
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-Processing
NMR software |
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NMR ensemble | Conformers calculated total number: 100 / Conformers submitted total number: 30 |