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Yorodumi- PDB-2crg: Solution structure of the myb-like DNA-binding domain of mouse MT... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2crg | ||||||
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Title | Solution structure of the myb-like DNA-binding domain of mouse MTA3 protein | ||||||
Components | Metastasis associated protein MTA3 | ||||||
Keywords | GENE REGULATION / transcription factor / helix turn helix / Structural Genomics / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information RNA Polymerase I Transcription Initiation / RNA polymerase II-specific DNA-binding transcription factor binding => GO:0061629 / HDACs deacetylate histones / Regulation of PTEN gene transcription / : / NuRD complex / positive regulation of G2/M transition of mitotic cell cycle / histone deacetylase binding / transcription corepressor activity / sequence-specific DNA binding ...RNA Polymerase I Transcription Initiation / RNA polymerase II-specific DNA-binding transcription factor binding => GO:0061629 / HDACs deacetylate histones / Regulation of PTEN gene transcription / : / NuRD complex / positive regulation of G2/M transition of mitotic cell cycle / histone deacetylase binding / transcription corepressor activity / sequence-specific DNA binding / transcription coactivator activity / intracellular membrane-bounded organelle / negative regulation of DNA-templated transcription / chromatin binding / positive regulation of cell population proliferation / protein-containing complex binding / negative regulation of transcription by RNA polymerase II / zinc ion binding / nucleoplasm / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Endo, H. / Hayashi, F. / Yoshida, M. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: To be published Title: Solution structure of the myb-like DNA-binding domain of mouse MTA3 protein Authors: Endo, H. / Hayashi, F. / Yoshida, M. / Yokoyama, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2crg.cif.gz | 419.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2crg.ent.gz | 367.5 KB | Display | PDB format |
PDBx/mmJSON format | 2crg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cr/2crg ftp://data.pdbj.org/pub/pdb/validation_reports/cr/2crg | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 8081.861 Da / Num. of mol.: 1 / Fragment: Myb_DNA_binding (residues 1-70) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Description: cell free protein synthesis / Gene: Mta3 / Plasmid: P041213-14 / References: UniProt: Q924K8 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.07mM 13C,15N-labeled protein; 20mM d-Tris-HCl(pH7.0); 100mM NaCl; 1mM d-DTT; 0.02% NaN3 Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 120 mM / pH: 7.0 / Pressure: ambient / Temperature: 293 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function, structures with the lowest energy, structures with the least restraint violations Conformers calculated total number: 100 / Conformers submitted total number: 20 |