SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED.
Mass: 18.015 Da / Num. of mol.: 6 / Source method: isolated from a natural source / Formula: H2O
Compound details
HAS A ROLE IN THE MATURATION OF 5S RRNA FROM ITS PRECURSORS FROM ALL THE RRNA GENES
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 7.1 Å3/Da / Density % sol: 82 %
Crystal grow
pH: 8 Details: CRYSTALLIZATION CONDITIONS: CRYSTALS OF THE RNASE E CATALYTIC DOMAIN/ RNA COMPLEX APPEARED AFTER TWO TO FOUR WEEKS IN 5 TO 20 % WT/V POLYETHYLENE GLYCOL 8,000, 0.1 M TRIS PH 7.5 TO 8.0, AND ...Details: CRYSTALLIZATION CONDITIONS: CRYSTALS OF THE RNASE E CATALYTIC DOMAIN/ RNA COMPLEX APPEARED AFTER TWO TO FOUR WEEKS IN 5 TO 20 % WT/V POLYETHYLENE GLYCOL 8,000, 0.1 M TRIS PH 7.5 TO 8.0, AND 10 TO 50 MM MAGNESIUM FORMATE AT 20OC
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Data collection
Diffraction
Mean temperature: 100 K
Diffraction source
Source: SYNCHROTRON / Site: ALS / Type: ALS / Wavelength: 1.1
Detector
Type: ADSC CCD / Detector: CCD
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 1.1 Å / Relative weight: 1
Reflection
Resolution: 3.6→25 Å / Num. obs: 19065 / % possible obs: 99.8 % / Observed criterion σ(I): 0 / Redundancy: 16 % / Rmerge(I) obs: 0.13 / Net I/σ(I): 23
Reflection shell
Resolution: 3.6→3.73 Å / Redundancy: 10 % / Rmerge(I) obs: 0.64 / Mean I/σ(I) obs: 209 / % possible all: 97.7
Resolution: 3.6→25 Å / Cor.coef. Fo:Fc: 0.862 / Cor.coef. Fo:Fc free: 0.838 / SU B: 42.384 / SU ML: 0.306 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / ESU R: 1.355 / ESU R Free: 0.584 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. DISORDERED REGIONS WERE REMOVED FROM THE MODEL
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.347
973
5.1 %
RANDOM
Rwork
0.319
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obs
0.32
17938
99.7 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK