SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 11-STRANDED BARREL THIS IS REPRESENTED BY A 12-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL. THE SHEETS PRESENTED AS "BA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 11-STRANDED BARREL THIS IS REPRESENTED BY A 12-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL.
Mass: 36434.523 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: SCHIFF BASE LINK BETWEEN LYS 200 AND DSB, SCHIFF BASE LINK BETWEEN LYS 253 AND DSB Source: (gene. exp.) CHLOROBIUM VIBRIOFORME (bacteria) / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: Q59334, porphobilinogen synthase
Resolution: 2.6→89.44 Å / Cor.coef. Fo:Fc: 0.926 / Cor.coef. Fo:Fc free: 0.877 / SU B: 17.322 / SU ML: 0.361 / Cross valid method: THROUGHOUT / ESU R Free: 0.476 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. THERE WAS NO ELECTRON DENSITY TO INDICATE THE POSITIONS OF RESIDUES 1 TO 9 SO THESE RESIDUES HAVE BEEN OMITTED FROM THE STRUCTURE.
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.32
980
5.2 %
RANDOM
Rwork
0.26
-
-
-
obs
0.263
17930
90 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK