Golgi-associated vesicle / cellular response to transforming growth factor beta stimulus / non-specific protein-tyrosine kinase / non-membrane spanning protein tyrosine kinase activity / protein autophosphorylation / non-specific serine/threonine protein kinase / RNA polymerase II cis-regulatory region sequence-specific DNA binding / protein serine/threonine kinase activity / perinuclear region of cytoplasm / positive regulation of transcription by RNA polymerase II ...Golgi-associated vesicle / cellular response to transforming growth factor beta stimulus / non-specific protein-tyrosine kinase / non-membrane spanning protein tyrosine kinase activity / protein autophosphorylation / non-specific serine/threonine protein kinase / RNA polymerase II cis-regulatory region sequence-specific DNA binding / protein serine/threonine kinase activity / perinuclear region of cytoplasm / positive regulation of transcription by RNA polymerase II / ATP binding / membrane / cytoplasm / cytosol Similarity search - Function
Transferase(Phosphotransferase) domain 1 / Transferase(Phosphotransferase); domain 1 / Phosphorylase Kinase; domain 1 / Phosphorylase Kinase; domain 1 / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Protein kinase domain profile. / Protein kinase domain ...Transferase(Phosphotransferase) domain 1 / Transferase(Phosphotransferase); domain 1 / Phosphorylase Kinase; domain 1 / Phosphorylase Kinase; domain 1 / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily / 2-Layer Sandwich / Orthogonal Bundle / Mainly Alpha / Alpha Beta Similarity search - Domain/homology
Mass: 18.015 Da / Num. of mol.: 76 / Source method: isolated from a natural source / Formula: H2O
Compound details
ENGINEERED RESIDUE IN CHAIN A, ARG 18 TO HIS ENGINEERED RESIDUE IN CHAIN B, ARG 18 TO HIS ...ENGINEERED RESIDUE IN CHAIN A, ARG 18 TO HIS ENGINEERED RESIDUE IN CHAIN B, ARG 18 TO HIS ENGINEERED RESIDUE IN CHAIN A, ARG 265 TO TRP ENGINEERED RESIDUE IN CHAIN B, ARG 265 TO TRP
Sequence details
MUTATION R18H AND R265W
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 2
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Sample preparation
Crystal
Density Matthews: 4.5 Å3/Da / Density % sol: 72.3 %
Crystal grow
Method: vapor diffusion, sitting drop Details: 200 NL SITTING DROP, 1.1 AMMONIUM SULFATE, 1% PEG3350, 0.1 M BIS-TRIS PH 5.0