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- PDB-2bty: Acetylglutamate kinase from Thermotoga maritima complexed with it... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2bty | |||||||||
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Title | Acetylglutamate kinase from Thermotoga maritima complexed with its inhibitor arginine | |||||||||
![]() | ACETYLGLUTAMATE KINASE![]() | |||||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Gil-Ortiz, F. / Fernandez-Murga, M.L. / Fita, I. / Rubio, V. | |||||||||
![]() | ![]() Title: Structural Bases of Feed-Back Control of Arginine Biosynthesis, Revealed by the Structure of Two Hexameric N-Acetylglutamate Kinases, from Thermotoga Maritima and Pseudomonas Aeruginosa Authors: Ramon-Maiques, S. / Fernandez-Murga, M.L. / Gil-Ortiz, F. / Vagin, A. / Fita, I. / Rubio, V. | |||||||||
History |
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Remark 650 | HELIX DETERMINATION METHOD: AUTHOR PROVIDED. | |||||||||
Remark 700 | SHEET DETERMINATION METHOD: AUTHOR PROVIDED. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 164.8 KB | Display | ![]() |
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PDB format | ![]() | 138.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 329.4 KB | Display | ![]() |
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Full document | ![]() | 362.4 KB | Display | |
Data in XML | ![]() | 36.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2bufC ![]() 1bufS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | ![]() Mass: 30385.623 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() ![]() #2: Chemical | ![]() #3: Chemical | ![]() #4: Chemical | ChemComp-K / | #5: Water | ChemComp-HOH / | ![]() Compound details | CATALYSES CONVERSION OF ATP AND N-ACETYL-L-GLUTAMATE TO ADP AND N-ACETYL-L-GLUTAMATE 5-PHOSPHATE. ...CATALYSES CONVERSION | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.2 Å3/Da / Density % sol: 61.2 % |
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Crystal grow![]() | pH: 4.6 Details: 15% POLYETHYLENE GLYCOL 4K, SODIUM ACETATE 50 MM PH 4.6, AMMONIUM SULFATE 75 MM |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Oct 22, 2001 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.75→20 Å / Num. obs: 30394 / % possible obs: 98.8 % / Observed criterion σ(I): 0 / Redundancy: 3.2 % / Biso Wilson estimate: 29.8 Å2 / Rmerge(I) obs: 0.06 / Net I/σ(I): 22 |
Reflection shell | Resolution: 2.75→2.85 Å / Redundancy: 3.1 % / Rmerge(I) obs: 0.36 / Mean I/σ(I) obs: 4.1 / % possible all: 98.4 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 1BUF Resolution: 2.75→19.95 Å / Rfactor Rfree error: 0.007 / Data cutoff high absF: 2182059.08 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 30.8586 Å2 / ksol: 0.324339 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 55.7 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.75→19.95 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.75→2.92 Å / Rfactor Rfree error: 0.023 / Total num. of bins used: 6
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Xplor file |
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