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- PDB-2brz: SOLUTION NMR STRUCTURE OF THE SWEET PROTEIN BRAZZEIN, MINIMIZED A... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2brz | |||||||||
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Title | SOLUTION NMR STRUCTURE OF THE SWEET PROTEIN BRAZZEIN, MINIMIZED AVERAGE STRUCTURE | |||||||||
![]() | BRAZZEIN | |||||||||
![]() | SWEET PROTEIN / CYSTEINE-STABILIZED ALPHA-BETA | |||||||||
Function / homology | ![]() defense response to fungus / killing of cells of another organism / defense response to bacterium / extracellular region Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | SOLUTION NMR / DYNAMICAL SIMULATED ANNEALING | |||||||||
![]() | Caldwell, J.E. / Abildgaard, F. / Dzakula, Z. / Ming, D. / Hellekant, G. / Markley, J.L. | |||||||||
![]() | ![]() Title: Solution structure of the thermostable sweet-tasting protein brazzein. Authors: Caldwell, J.E. / Abildgaard, F. / Dzakula, Z. / Ming, D. / Hellekant, G. / Markley, J.L. #1: ![]() Title: Complete 1H and Partial 13C Resonance Assignments at 37 and 22 Degrees C for Brazzein, an Intensely Sweet Protein Authors: Caldwell, J.E. / Abildgaard, F. / Ming, D. / Hellekant, G. / Markley, J.L. #2: ![]() Title: Brazzein, a New High-Potency Thermostable Sweet Protein from Pentadiplandra Brazzeana B Authors: Ming, D. / Hellekant, G. | |||||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 23.6 KB | Display | ![]() |
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PDB format | ![]() | 17.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 337.6 KB | Display | ![]() |
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Full document | ![]() | 341.6 KB | Display | |
Data in XML | ![]() | 2.9 KB | Display | |
Data in CIF | ![]() | 3.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 6491.328 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: EXTRACTED FROM FRUIT GROWN IN THE WILD / Source: (natural) ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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Sample preparation
Sample conditions | pH: 5.2 / Temperature: 295 K |
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Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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Processing
Software |
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NMR software |
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Refinement | Method: DYNAMICAL SIMULATED ANNEALING / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. REFINED USING FOUR CYCLES OF SIMULATED ANNEALING REFINEMENT IN X-PLOR. | ||||||||||||
NMR ensemble | Conformer selection criteria: MINIMIZED AVERAGE / Conformers calculated total number: 1 / Conformers submitted total number: 1 |