SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED.
Mass: 18.015 Da / Num. of mol.: 55 / Source method: isolated from a natural source / Formula: H2O
Compound details
FUNCTION: TRANSFER OF TWO REDUCING EQUIVALENTS IN A FORM OF THE HYDRIDE ION FROM NADPH VIA FAD AND ...FUNCTION: TRANSFER OF TWO REDUCING EQUIVALENTS IN A FORM OF THE HYDRIDE ION FROM NADPH VIA FAD AND FMN TO CYTOCHROME P450 IN ONE-ELECTRON TRANSFER STEP
Sequence details
RESIDUES NUMBERING IN THE ENTRY BEGINS FROM THE START METHIONINE 33 N-TERMINUS RESIDUES ARE ...RESIDUES NUMBERING IN THE ENTRY BEGINS FROM THE START METHIONINE 33 N-TERMINUS RESIDUES ARE TRUNCATED, 6XHIS RESIDUES AT THE N-TERMINUS ARE PRECEDED BY 4 AND FOLLOWED BY 15 RESIDUES FROM THE CLONING SITE.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.58 Å3/Da / Density % sol: 51 %
Crystal grow
Temperature: 293 K / pH: 5 Details: 1.6 M AMMONIUM SULFATE 100 MM SODIUM CITRATE (PH 5.0) 1 MM FAD 1 MM NADPH T=20 C
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