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Open data
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Basic information
Entry | Database: PDB / ID: 2bjh | |||||||||
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Title | Crystal Structure Of S133A AnFaeA-ferulic acid complex | |||||||||
![]() | FERULOYL ESTERASE A | |||||||||
![]() | HYDROLASE / DEGRADATION PLANT CELL WALLS / FERULOYL ESTERASE / GLYCOPROTEIN / SERINE ESTERASE / XYLAN DEGRADATION | |||||||||
Function / homology | ![]() feruloyl esterase / feruloyl esterase activity / pectin catabolic process / cellulose binding / xylan catabolic process / cellulose catabolic process / lipid metabolic process / cell wall macromolecule catabolic process / extracellular region Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Faulds, C.B. / Molina, R. / Gonzalez, R. / Husband, F. / Juge, N. / Sanz-Aparicio, J. / Hermoso, J.A. | |||||||||
![]() | ![]() Title: Probing the Determinants of Substrate Specificity of a Feruloyl Esterase, Anfaea, from Aspergillus Niger Authors: Faulds, C.B. / Molina, R. / Gonzalez, R. / Husband, F. / Juge, N. / Sanz-Aparicio, J. / Hermoso, J.A. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 169.3 KB | Display | ![]() |
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PDB format | ![]() | 134.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 492.8 KB | Display | ![]() |
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Full document | ![]() | 527.3 KB | Display | |
Data in XML | ![]() | 38.3 KB | Display | |
Data in CIF | ![]() | 52.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1uswS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 28343.875 Da / Num. of mol.: 3 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Sugar | #3: Sugar | #4: Chemical | #5: Water | ChemComp-HOH / | Compound details | INVOLVED IN DEGRADATION OF PLANT CELL WALLS. HYDROLYZES THE FERULOYL-ARABINOSE ESTER BOND IN ...INVOLVED IN DEGRADATIO | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.72 Å3/Da / Density % sol: 54.81 % |
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Crystal grow | pH: 7.5 Details: 1.7 M AMMONIUM SULPHATE, 0.1 M HEPES, PH = 7.5, [FAXX]= 10 MM. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Oct 14, 2003 / Details: MIRRORS |
Radiation | Monochromator: DIAMOND / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.918381 Å / Relative weight: 1 |
Reflection | Resolution: 2.15→49.5 Å / Num. obs: 47613 / % possible obs: 98.6 % / Redundancy: 3.1 % / Biso Wilson estimate: 30.5 Å2 / Rmerge(I) obs: 0.2 / Net I/σ(I): 5.9 |
Reflection shell | Resolution: 2.41→2.58 Å / Redundancy: 3.1 % / Rmerge(I) obs: 0.5 / Mean I/σ(I) obs: 2.3 / % possible all: 99.4 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1USW Resolution: 2.54→49.49 Å / Rfactor Rfree error: 0.006 / Data cutoff high absF: 1573406.34 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 44.0691 Å2 / ksol: 0.376446 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 29.1 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.54→49.49 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.54→2.7 Å / Rfactor Rfree error: 0.02 / Total num. of bins used: 6
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Xplor file |
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