THIS ENTRY CONTAINS ONE OF NINE STRUCTURES THAT ARE DESCRIBED IN THE ACCOMPANYING JOURNAL ARTICLE. ...THIS ENTRY CONTAINS ONE OF NINE STRUCTURES THAT ARE DESCRIBED IN THE ACCOMPANYING JOURNAL ARTICLE. IN THE ARTICLE THE STRUCTURES ARE REFERRED TO BY LETTER, RATHER THAN BY PDB ACCESSION CODE. THE MAPPING BETWEEN LABEL AND ACCESSION CODE IS AS FOLLOWS: A - 2BHX F - 2BI9 B - 2BI1 G - 2BIA C - 2BI2 H - 2BIE D - 2BI3 I - 2BIG E - 2BI5
配列の詳細
THE N-TERMINAL METHIONINE IS CLEAVED OFF IN THE MATURE PROTEIN
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 2.13 Å3/Da / 溶媒含有率: 42.3 %
結晶化
pH: 8.2 詳細: 30% PEG 400, 200 MM MGCL2, 5% GLYCEROL, 100 MM TRIS PH 8.2
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 0.939 Å / 相対比: 1
反射
解像度: 1.69→15 Å / Num. obs: 90981 / % possible obs: 98.7 % / Observed criterion σ(I): 3 / Rmerge(I) obs: 0.08 / Net I/σ(I): 11
反射 シェル
解像度: 1.69→1.73 Å / Rmerge(I) obs: 0.55 / Mean I/σ(I) obs: 2 / % possible all: 99.8
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解析
ソフトウェア
名称
分類
SHELXL-97
精密化
HKL
データ削減
HKL
データスケーリング
精密化
構造決定の手法: OTHER / 解像度: 1.69→15 Å / Num. parameters: 25439 / Num. restraintsaints: 23526 / 交差検証法: FREE R-VALUE / σ(F): 0 / 立体化学のターゲット値: ENGH AND HUBER 詳細: RESIDUES A 1, A 215, A 216, A 218, B 2 AND B 218 WERE MODELLED AS ALANINES. RESIDUES B 1, B 214, B 215, B216 WERE NOT MODELLED.
Rfactor
反射数
%反射
Selection details
Rfree
0.2293
4472
5 %
RANDOM
all
0.1808
89142
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obs
0.1793
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98.7 %
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Refine analyze
Num. disordered residues: 22 / Occupancy sum hydrogen: 0 / Occupancy sum non hydrogen: 6278