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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 2bgr | |||||||||
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タイトル | Crystal structure of HIV-1 Tat derived nonapeptides Tat(1-9) bound to the active site of Dipeptidyl peptidase IV (CD26) | |||||||||
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![]() | HYDROLASE / HYDROLASE-COMPLEX / DPPIV / ALPHA/BETA-HYDROLASE FOLD / BETA-PROPELLER FOLD | |||||||||
機能・相同性 | ![]() viral gene expression / trans-activation response element binding / regulatory region RNA binding / positive regulation of viral transcription / protein serine/threonine phosphatase inhibitor activity / glucagon processing / modulation by virus of host chromatin organization / negative regulation of neutrophil chemotaxis / symbiont-mediated suppression of host translation initiation / regulation of cell-cell adhesion mediated by integrin ...viral gene expression / trans-activation response element binding / regulatory region RNA binding / positive regulation of viral transcription / protein serine/threonine phosphatase inhibitor activity / glucagon processing / modulation by virus of host chromatin organization / negative regulation of neutrophil chemotaxis / symbiont-mediated suppression of host translation initiation / regulation of cell-cell adhesion mediated by integrin / Synthesis, secretion, and inactivation of Glucose-dependent Insulinotropic Polypeptide (GIP) / negative regulation of extracellular matrix disassembly / psychomotor behavior / dipeptidyl-peptidase IV / host cell nucleolus / intercellular canaliculus / chemorepellent activity / actinin binding / dipeptidyl-peptidase activity / peptide hormone processing / negative regulation of peptidyl-threonine phosphorylation / locomotory exploration behavior / lamellipodium membrane / endocytic vesicle / endothelial cell migration / behavioral fear response / RNA-binding transcription regulator activity / aminopeptidase activity / T cell costimulation / T cell activation / serine-type peptidase activity / cyclin binding / positive regulation of transcription elongation by RNA polymerase II / Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) / lamellipodium / virus receptor activity / protease binding / host cell cytoplasm / receptor-mediated endocytosis of virus by host cell / membrane fusion / receptor-mediated virion attachment to host cell / cell adhesion / response to hypoxia / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / symbiont entry into host cell / membrane raft / apical plasma membrane / protein domain specific binding / lysosomal membrane / serine-type endopeptidase activity / focal adhesion / signaling receptor binding / virus-mediated perturbation of host defense response / DNA-templated transcription / positive regulation of cell population proliferation / cell surface / protein homodimerization activity / proteolysis / extracellular exosome / extracellular region / identical protein binding / membrane / metal ion binding / plasma membrane 類似検索 - 分子機能 | |||||||||
生物種 | ![]() ![]() ![]() | |||||||||
手法 | ![]() ![]() ![]() | |||||||||
![]() | Weihofen, W.A. / Liu, J. / Reutter, W. / Saenger, W. / Fan, H. | |||||||||
![]() | ![]() タイトル: Crystal Structures of HIV-1 Tat-Derived Nonapeptides Tat-(1-9) and Trp2-Tat-(1-9) Bound to the Active Site of Dipeptidyl-Peptidase Iv (Cd26) 著者: Weihofen, W.A. / Liu, J. / Reutter, W. / Saenger, W. / Fan, H. | |||||||||
履歴 |
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Remark 700 | SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED. |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 352.2 KB | 表示 | ![]() |
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PDB形式 | ![]() | 284.3 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 3.6 MB | 表示 | ![]() |
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文書・詳細版 | ![]() | 3.6 MB | 表示 | |
XML形式データ | ![]() | 32.4 KB | 表示 | |
CIF形式データ | ![]() | 57.5 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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非結晶学的対称性 (NCS) | NCSドメイン:
NCSドメイン領域: Ens-ID: 1 / End auth comp-ID: SER / End label comp-ID: SER / Refine code: 6
NCS oper: (Code: given Matrix: (-0.946, 0.152, -0.287), ベクター: |
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要素
-タンパク質 / タンパク質・ペプチド / 非ポリマー , 3種, 1750分子 ABYZ![](data/chem/img/HOH.gif)
![](data/chem/img/HOH.gif)
#1: タンパク質 | 分子量: 85452.570 Da / 分子数: 2 / 断片: EXTRACELLULAR DOMAIN, RESIDUES 29-766 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() 発現宿主: ![]() ![]() 株 (発現宿主): SF9 CELLS / 参照: UniProt: P27487, dipeptidyl-peptidase IV #2: タンパク質・ペプチド | 分子量: 1029.121 Da / 分子数: 2 / 由来タイプ: 合成 由来: (合成) ![]() ![]() 参照: UniProt: P12506 #8: 水 | ChemComp-HOH / | |
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-糖 , 5種, 14分子 ![](data/chem/img/NAG.gif)
#3: 多糖 | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta- ...2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | ||||||
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#4: 多糖 | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose #5: 多糖 | #6: 多糖 | alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose | #7: 糖 | ChemComp-NAG / |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.61 Å3/Da / 溶媒含有率: 52.56 % |
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-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: ADSC CCD / 検出器: CCD / 日付: 2004年5月3日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.933 Å / 相対比: 1 |
反射 | 解像度: 2→30 Å / Num. obs: 127470 / % possible obs: 92 % / Observed criterion σ(I): 2 / 冗長度: 4.3 % / Rmerge(I) obs: 0.07 / Net I/σ(I): 19.8 |
反射 シェル | 解像度: 2→2.07 Å / 冗長度: 4 % / Rmerge(I) obs: 0.37 / Mean I/σ(I) obs: 3.1 / % possible all: 87.7 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: PDB ENTRY 1N1M 解像度: 2→30 Å / Cor.coef. Fo:Fc: 0.968 / Cor.coef. Fo:Fc free: 0.955 / SU B: 6.392 / SU ML: 0.095 / TLS residual ADP flag: LIKELY RESIDUAL / 交差検証法: THROUGHOUT / σ(F): 2 / ESU R: 0.147 / ESU R Free: 0.139 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. RESIDUES 29 - 37 ARE DISORDERED AND WERE NOT MODELLED
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溶媒の処理 | イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 38.7 Å2
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精密化ステップ | サイクル: LAST / 解像度: 2→30 Å
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拘束条件 |
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