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Yorodumi- PDB-2b87: Structural basis for molecular recognition in an affibody:affibod... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2b87 | ||||||
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Title | Structural basis for molecular recognition in an affibody:affibody complex | ||||||
Components |
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Keywords | PROTEIN BINDING / PROTEIN-PROTEIN INTERACTIONS PROTEIN ENGINEERING / MOLECULAR RECOGNITION / NMR SPECTROSCOPY / INDUCED FIT / AFFIBODY | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Staphylococcus aureus (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Lendel, C. / Dogan, J. / Hard, T. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2006 Title: Structural basis for molecular recognition in an affibody:affibody complex. Authors: Lendel, C. / Dogan, J. / Hard, T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2b87.cif.gz | 1.4 MB | Display | PDBx/mmCIF format |
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PDB format | pdb2b87.ent.gz | 1.2 MB | Display | PDB format |
PDBx/mmJSON format | 2b87.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2b87_validation.pdf.gz | 422 KB | Display | wwPDB validaton report |
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Full document | 2b87_full_validation.pdf.gz | 900.1 KB | Display | |
Data in XML | 2b87_validation.xml.gz | 119.8 KB | Display | |
Data in CIF | 2b87_validation.cif.gz | 161.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b8/2b87 ftp://data.pdbj.org/pub/pdb/validation_reports/b8/2b87 | HTTPS FTP |
-Related structure data
Related structure data | 2b88C 2b89C C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 6491.255 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Staphylococcus aureus (bacteria) / Plasmid: PET28A(+) / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 DE3 / References: UniProt: Q70AB8 |
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#2: Protein | Mass: 6427.346 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Staphylococcus aureus (bacteria) / Plasmid: PET28A(+) / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 DE3 / References: UniProt: Q70AB8 |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations, structures with the lowest energy, good ramachandran plots Conformers calculated total number: 100 / Conformers submitted total number: 40 |