+Open data
-Basic information
Entry | Database: PDB / ID: 2b5r | ||||||
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Title | 1B Lactamase / B Lactamase Inhibitor | ||||||
Components |
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Keywords | HYDROLASE/HYDROLASE INHIBITOR / Protein-Protein complex / Structural Genomics / Israel Structural Proteomics Center / ISPC / HYDROLASE-HYDROLASE INHIBITOR COMPLEX | ||||||
Function / homology | Function and homology information regulation of beta-lactamase activity / beta-lactam antibiotic catabolic process / beta-lactamase activity / beta-lactamase / response to antibiotic / extracellular region Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) Streptomyces clavuligerus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.65 Å | ||||||
Authors | Rahat, O. / Albeck, S. / Meged, R. / Dym, O. / Screiber, G. / Israel Structural Proteomics Center (ISPC) | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2006 Title: Binding Hot Spots in the TEM1-BLIP Interface in Light of its Modular Architecture. Authors: Reichmann, D. / Cohen, M. / Abramovich, R. / Dym, O. / Lim, D. / Strynadka, N.C. / Schreiber, G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2b5r.cif.gz | 178.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2b5r.ent.gz | 145.9 KB | Display | PDB format |
PDBx/mmJSON format | 2b5r.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b5/2b5r ftp://data.pdbj.org/pub/pdb/validation_reports/b5/2b5r | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 28941.010 Da / Num. of mol.: 2 / Mutation: V84I, E104Y, Y105N Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: bla / Production host: Escherichia coli (E. coli) / References: UniProt: P62593, beta-lactamase #2: Protein | Mass: 17556.492 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptomyces clavuligerus (bacteria) / Production host: Escherichia coli (E. coli) / References: UniProt: P35804 #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.27 Å3/Da / Density % sol: 46 % |
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Crystal grow | Temperature: 298 K / pH: 8.5 Details: PEG 3350, NH4 Acetate, pH 8.5, Microbatch, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-4 / Wavelength: 0.97565 Å |
Detector | Type: NONIUS CAD4 / Detector: CCD / Date: Jul 1, 2005 |
Radiation | Monochromator: Yale mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97565 Å / Relative weight: 1 |
Reflection | Resolution: 1.65→50 Å / Num. all: 109217 / Num. obs: 109217 / % possible obs: 100 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 7 % / Biso Wilson estimate: 18 Å2 / Rmerge(I) obs: 0.075 / Rsym value: 0.065 / Net I/σ(I): 18.5 |
Reflection shell | Resolution: 1.65→1.68 Å / Redundancy: 7 % / Rmerge(I) obs: 0.348 / Mean I/σ(I) obs: 4 / Num. unique all: 5385 / Rsym value: 0.329 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.65→48.3 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.65→48.3 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.65→1.75 Å
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