+Open data
-Basic information
Entry | Database: PDB / ID: 2b58 | ||||||
---|---|---|---|---|---|---|---|
Title | SSAT with coa_sp, spermine disordered, K26R mutant | ||||||
Components | Diamine acetyltransferase 1 | ||||||
Keywords | TRANSFERASE / Structural Genomics / PSI / Protein Structure Initiative / New York SGX Research Center for Structural Genomics / NYSGXRC | ||||||
Function / homology | Function and homology information Interconversion of polyamines / spermidine acetylation / spermidine binding / putrescine catabolic process / polyamine biosynthetic process / diamine N-acetyltransferase / diamine N-acetyltransferase activity / N-acetyltransferase activity / angiogenesis / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SAD / Resolution: 1.95 Å | ||||||
Authors | Bewley, M.C. / Graziano, V. / Jiang, J.S. / Matz, E. / Studier, F.W. / Pegg, A.P. / Coleman, C.S. / Flanagan, J.M. / Burley, S.K. / New York SGX Research Center for Structural Genomics (NYSGXRC) | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.Usa / Year: 2006 Title: Structures of wild-type and mutant human spermidine/spermine N1-acetyltransferase, a potential therapeutic drug target Authors: Bewley, M.C. / Graziano, V. / Jiang, J.S. / Matz, E. / Studier, F.W. / Pegg, A.P. / Coleman, C.S. / Flanagan, J.M. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2b58.cif.gz | 45.9 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2b58.ent.gz | 36.4 KB | Display | PDB format |
PDBx/mmJSON format | 2b58.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2b58_validation.pdf.gz | 707.2 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 2b58_full_validation.pdf.gz | 711.7 KB | Display | |
Data in XML | 2b58_validation.xml.gz | 10.7 KB | Display | |
Data in CIF | 2b58_validation.cif.gz | 13.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b5/2b58 ftp://data.pdbj.org/pub/pdb/validation_reports/b5/2b58 | HTTPS FTP |
-Related structure data
Related structure data | 2b3uC 2b3vC 2b4bC 2b4dC 2b5gC 2b47 C: citing same article (ref.) |
---|---|
Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 20404.268 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: SAT / Plasmid: pET13a / Production host: Escherichia coli (E. coli) / References: UniProt: P21673, diamine N-acetyltransferase |
---|---|
#2: Chemical | ChemComp-COA / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
---|
-Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.41 % |
---|
-Data collection
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
---|---|
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 1.95→30 Å / Num. all: 13447 / Num. obs: 13228 |
-Processing
Software | Name: CNS / Version: 1.1 / Classification: refinement | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: SAD / Resolution: 1.95→30 Å / σ(F): -3
| |||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.95→30 Å
|