+Open data
-Basic information
Entry | Database: PDB / ID: 2az2 | ||||||
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Title | Flock House virus B2-dsRNA Complex (P4122) | ||||||
Components |
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Keywords | Viral protein/RNA / protein-rna complex / four-helix bundle / Viral protein-RNA COMPLEX | ||||||
Function / homology | Function and homology information host cell mitochondrion / virus-mediated perturbation of host defense response / RNA binding Similarity search - Function | ||||||
Biological species | Flock house virus | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.6 Å | ||||||
Authors | Chao, J.A. / Lee, J.H. / Chapados, B.R. / Debler, E.W. / Schneemann, A. / Williamson, J.R. | ||||||
Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2005 Title: Dual modes of RNA-silencing suppression by Flock House virus protein B2. Authors: Chao, J.A. / Lee, J.H. / Chapados, B.R. / Debler, E.W. / Schneemann, A. / Williamson, J.R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2az2.cif.gz | 59.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2az2.ent.gz | 42.1 KB | Display | PDB format |
PDBx/mmJSON format | 2az2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/az/2az2 ftp://data.pdbj.org/pub/pdb/validation_reports/az/2az2 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: RNA chain | Mass: 6000.171 Da / Num. of mol.: 2 / Source method: obtained synthetically #2: Protein | Mass: 8036.355 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Flock house virus / Genus: Alphanodavirus / Gene: B2 / Production host: Escherichia coli (E. coli) / References: UniProt: P68831 #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3.34 Å3/Da / Density % sol: 63.14 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 7 Details: MPEG 5K, ammomnium sulfate, sodium chloride, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 93 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL11-1 / Wavelength: 0.9191 Å |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Jul 15, 2005 |
Radiation | Monochromator: graphite / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9191 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→35.6 Å / Num. obs: 12367 / % possible obs: 100 % / Observed criterion σ(F): 0.008 / Observed criterion σ(I): 2 |
Reflection shell | Resolution: 2.6→2.74 Å / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.6→35.6 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.6→35.6 Å
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Refine LS restraints |
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