+Open data
-Basic information
Entry | Database: PDB / ID: 2amg | |||||||||
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Title | STRUCTURE OF HYDROLASE (GLYCOSIDASE) | |||||||||
Components | 1,4-ALPHA-D-GLUCAN MALTOTETRAHYDROLASE | |||||||||
Keywords | HYDROLASE / GLYCOSIDASE / CARBOHYDRATE METABOLISM | |||||||||
Function / homology | Function and homology information glucan 1,4-alpha-maltotetraohydrolase / glucan 1,4-alpha-maltotetraohydrolase activity / starch catabolic process / starch binding / alpha-amylase activity / extracellular space / metal ion binding Similarity search - Function | |||||||||
Biological species | Pseudomonas stutzeri (bacteria) | |||||||||
Method | X-RAY DIFFRACTION / Resolution: 2 Å | |||||||||
Authors | Morishita, Y. / Hasegawa, K. / Matsuura, Y. / Kubota, M. / Sakai, S. / Katsube, Y. | |||||||||
Citation | Journal: J.Mol.Biol. / Year: 1997 Title: Crystal structure of a maltotetraose-forming exo-amylase from Pseudomonas stutzeri. Authors: Morishita, Y. / Hasegawa, K. / Matsuura, Y. / Katsube, Y. / Kubota, M. / Sakai, S. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2amg.cif.gz | 96.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2amg.ent.gz | 73.6 KB | Display | PDB format |
PDBx/mmJSON format | 2amg.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2amg_validation.pdf.gz | 366.9 KB | Display | wwPDB validaton report |
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Full document | 2amg_full_validation.pdf.gz | 380.6 KB | Display | |
Data in XML | 2amg_validation.xml.gz | 11.1 KB | Display | |
Data in CIF | 2amg_validation.cif.gz | 17.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/am/2amg ftp://data.pdbj.org/pub/pdb/validation_reports/am/2amg | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 46581.824 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Pseudomonas stutzeri (bacteria) References: UniProt: P13507, glucan 1,4-alpha-maltotetraohydrolase | ||
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#2: Chemical | #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.8 Å3/Da / Density % sol: 56.1 % | |||||||||||||||||||||||||
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Crystal | *PLUS Density % sol: 44 % | |||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / pH: 7.5 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Detector | Type: RIGAKU / Detector: IMAGE PLATE |
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Radiation | Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2→10 Å / Num. obs: 33397 / % possible obs: 91.6 % / Observed criterion σ(I): 1 / Redundancy: 4.1 % / Rmerge(I) obs: 0.066 |
Reflection | *PLUS Num. measured all: 138001 |
-Processing
Software |
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Refinement | Resolution: 2→10 Å / σ(F): 2 / Details: X-PLOR ALSO WAS USED.
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Refine analyze | Luzzati coordinate error obs: 0.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→10 Å
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Refine LS restraints |
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Software | *PLUS Name: PROFFT / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS σ(I): 1 / Rfactor obs: 0.178 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |