- PDB-2ajo: NMR structure of the in-plane membrane anchor domain [1-28] of th... -
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Basic information
Entry
Database: PDB / ID: 2ajo
Title
NMR structure of the in-plane membrane anchor domain [1-28] of the monotopic NonStructural Protein 5A (NS5A) from the Bovine Viral Diarrhea Virus (BVDV)
Components
Nonstructural protein 5A
Keywords
MEMBRANE PROTEIN / in-plane membrane anchor domain / amphipathic alpha-helix
Function / homology
Function and homology information
pestivirus NS3 polyprotein peptidase / ribonuclease T2 / serine-type exopeptidase activity / ribonuclease T2 activity / nucleoside-triphosphate phosphatase / protein complex oligomerization / monoatomic ion channel activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity / clathrin-dependent endocytosis of virus by host cell / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases ...pestivirus NS3 polyprotein peptidase / ribonuclease T2 / serine-type exopeptidase activity / ribonuclease T2 activity / nucleoside-triphosphate phosphatase / protein complex oligomerization / monoatomic ion channel activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity / clathrin-dependent endocytosis of virus by host cell / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases / RNA helicase activity / host cell endoplasmic reticulum membrane / viral protein processing / RNA helicase / induction by virus of host autophagy / RNA-directed RNA polymerase / viral RNA genome replication / cysteine-type endopeptidase activity / serine-type endopeptidase activity / RNA-dependent RNA polymerase activity / virus-mediated perturbation of host defense response / fusion of virus membrane with host endosome membrane / virion attachment to host cell / GTP binding / virion membrane / ATP hydrolysis activity / proteolysis / RNA binding / ATP binding / membrane Similarity search - Function
Mass: 3204.788 Da / Num. of mol.: 1 / Fragment: BVDV NS5A / Source method: obtained synthetically Details: The peptide was chemically synthesized. The sequence is naturally found in Bovine Viral Diarrhea Virus 1, strain CP7 References: UniProt: Q96662
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
2D TOCSY
1
2
1
2D NOESY
1
3
1
1H 13C HSQC
1
4
1
DQF-COSY
2
5
1
2D TOCSY
2
6
1
1H 13C HSQC
2
7
1
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Sample preparation
Details
Contents: 2 mM BVDV NS5A[1-28], 100 mM SDS-d25 in H2O / Solvent system: 100 mM SDS-d25
Sample conditions
Conditions-ID
Ionic strength
pH
Pressure (kPa)
Temperature (K)
1
null
4.5
ambient
303K
2
null
4.5
ambient
313K
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NMR measurement
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M
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