+Open data
-Basic information
Entry | Database: PDB / ID: 2a1c | ||||||
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Title | Solution structure of CSP1 | ||||||
Components | CSP1 | ||||||
Keywords | HORMONE/GROWTH FACTOR / a-helix / HORMONE-GROWTH FACTOR COMPLEX | ||||||
Function / homology | Competence protein, ComC / COMC family / pheromone activity / establishment of competence for transformation / extracellular region / Competence-stimulating peptide type 1 Function and homology information | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Johnsborg, O. / Kristiansen, P.E. | ||||||
Citation | Journal: To be Published Title: A Hydrophobic Patch in the Pneumococcal Competence Pheromone CSP is Essential for Specificity and Biological Activity Authors: Johnsborg, O. / Kristiansen, P.E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2a1c.cif.gz | 113.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2a1c.ent.gz | 90.3 KB | Display | PDB format |
PDBx/mmJSON format | 2a1c.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a1/2a1c ftp://data.pdbj.org/pub/pdb/validation_reports/a1/2a1c | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 2248.735 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: The peptide was chemically synthesized. This sequence occures naturally. References: UniProt: P60243*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.9mM CSP1 0.1% TFA, 90% H2O, 10% D2O, 250mM D38 DPC Solvent system: 90% H20, 10% D2O, 250mM D38 DPC |
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Sample conditions | Ionic strength: 0 / pH: 5.2 / Pressure: ambient / Temperature: 301 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |