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Open data
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Basic information
Entry | Database: PDB / ID: 2a1c | ||||||
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Title | Solution structure of CSP1 | ||||||
![]() | CSP1 | ||||||
![]() | HORMONE/GROWTH FACTOR / a-helix / HORMONE-GROWTH FACTOR COMPLEX | ||||||
Function / homology | Competence protein, ComC / COMC family / pheromone activity / establishment of competence for transformation / extracellular region / Competence-stimulating peptide type 1![]() | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
![]() | Johnsborg, O. / Kristiansen, P.E. | ||||||
![]() | ![]() Title: A Hydrophobic Patch in the Pneumococcal Competence Pheromone CSP is Essential for Specificity and Biological Activity Authors: Johnsborg, O. / Kristiansen, P.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 115.4 KB | Display | ![]() |
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PDB format | ![]() | 80.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 331.1 KB | Display | ![]() |
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Full document | ![]() | 448.2 KB | Display | |
Data in XML | ![]() | 7.5 KB | Display | |
Data in CIF | ![]() | 11.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 2248.735 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: The peptide was chemically synthesized. This sequence occures naturally. References: UniProt: P60243*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 1.9mM CSP1 0.1% TFA, 90% H2O, 10% D2O, 250mM D38 DPC Solvent system: 90% H20, 10% D2O, 250mM D38 DPC |
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Sample conditions | Ionic strength: 0 / pH: 5.2 / Pressure: ambient / Temperature: 301 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz |
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Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |