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Yorodumi- PDB-1zwv: Solution Structure of the subunit binding domain (hbSBD) of the h... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1zwv | ||||||
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Title | Solution Structure of the subunit binding domain (hbSBD) of the human mitochondrial branched-chain alpha-ketoacid dehydrogenase | ||||||
Components | Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial | ||||||
Keywords | TRANSFERASE / subunit binding domain | ||||||
Function / homology | Function and homology information dihydrolipoyllysine-residue (2-methylpropanoyl)transferase / dihydrolipoyllysine-residue (2-methylpropanoyl)transferase activity / : / lipoic acid binding / branched-chain amino acid catabolic process / Branched-chain amino acid catabolism / Glyoxylate metabolism and glycine degradation / mitochondrial nucleoid / acetyltransferase activity / RHOH GTPase cycle ...dihydrolipoyllysine-residue (2-methylpropanoyl)transferase / dihydrolipoyllysine-residue (2-methylpropanoyl)transferase activity / : / lipoic acid binding / branched-chain amino acid catabolic process / Branched-chain amino acid catabolism / Glyoxylate metabolism and glycine degradation / mitochondrial nucleoid / acetyltransferase activity / RHOH GTPase cycle / microtubule cytoskeleton / mitochondrial matrix / ubiquitin protein ligase binding / mitochondrion / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR | ||||||
Authors | Chang, C.F. / Chuang, D.T. / Huang, T.h. | ||||||
Citation | Journal: to be published Title: Solution Structure of the subunit binding domain (hbSBD) of the human mitochondrial branched-chain alpha-ketoacid dehydrogenase Authors: Chang, C.F. / Chuang, D.T. / Huang, T.h. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1zwv.cif.gz | 377.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1zwv.ent.gz | 330 KB | Display | PDB format |
PDBx/mmJSON format | 1zwv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zw/1zwv ftp://data.pdbj.org/pub/pdb/validation_reports/zw/1zwv | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 6635.617 Da / Num. of mol.: 1 / Fragment: E1/E3 subunit binding domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Plasmid: pET28a / Production host: Escherichia coli (E. coli) References: UniProt: P11182, dihydrolipoyllysine-residue (2-methylpropanoyl)transferase |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.5mM w/wo U-15N, 13C; 50mM Phosphate buffer / Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 100mM NaCl / pH: 7.5 / Pressure: ambient / Temperature: 295 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20 |