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Yorodumi- PDB-1zno: Crystal Structure of VC702 from Vibrio Cholerae, Northeast Struct... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1zno | ||||||
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Title | Crystal Structure of VC702 from Vibrio Cholerae, Northeast Structural Genomics Consortium Target: VcP1 | ||||||
Components | Hypothetical UPF0244 protein VC0702 | ||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / mixed alpha/beta sandwich / homodimer / PSI / Protein Structure Initiative / Northeast Structural Genomics Consortium / NESG | ||||||
Function / homology | Function and homology information inosine/xanthosine triphosphatase / ITPase activity / thiamine metabolic process / nucleotide metabolic process / ribonucleoside triphosphate phosphatase activity / nucleotide binding / metal ion binding Similarity search - Function | ||||||
Biological species | Vibrio cholerae (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2 Å | ||||||
Authors | Ni, S. / Forouhar, F. / Bussiere, D.E. / Robinson, H. / Kennedy, M.A. / Northeast Structural Genomics Consortium (NESG) | ||||||
Citation | Journal: Proteins / Year: 2006 Title: Crystal structure of VC0702 at 2.0 A: conserved hypothetical protein from Vibrio cholerae. Authors: Ni, S. / Forouhar, F. / Bussiere, D.E. / Robinson, H. / Kennedy, M.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1zno.cif.gz | 77.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1zno.ent.gz | 62.9 KB | Display | PDB format |
PDBx/mmJSON format | 1zno.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zn/1zno ftp://data.pdbj.org/pub/pdb/validation_reports/zn/1zno | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 20869.975 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Vibrio cholerae (bacteria) / Gene: vibrio cholerae / Plasmid: pET28 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21DE3 pLysS / References: UniProt: Q9KU27 #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.1 Å3/Da / Density % sol: 40.7 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 30% Peg 4000, .1M TRis pH 8.5, .2M MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X12C / Wavelength: 0.97912 Å |
Detector | Type: BRANDEIS - B4 / Detector: CCD / Date: Jun 23, 2003 |
Radiation | Monochromator: double crystal monochromater Si(111), beam focused by toroidal mirror Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97912 Å / Relative weight: 1 |
Reflection | Resolution: 2→30 Å / Num. all: 23994 / Num. obs: 23994 / % possible obs: 99.9 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 |
Reflection shell | Resolution: 2→2.07 Å / % possible all: 99.6 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 2→30 Å / σ(F): 2 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2→30 Å
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Refine LS restraints |
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