+Open data
-Basic information
Entry | Database: PDB / ID: 1zj6 | ||||||
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Title | Crystal structure of human ARL5 | ||||||
Components | ADP-ribosylation factor-like protein 5 | ||||||
Keywords | TRANSPORT PROTEIN / ARL / GTP-binding | ||||||
Function / homology | Function and homology information protein localization to Golgi membrane / vesicle-mediated transport / intracellular protein transport / trans-Golgi network / GTPase activity / GTP binding Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Wang, Z.X. / Shi, L. / Liu, J.F. / An, X.M. / Chang, W.R. / Liang, D.C. | ||||||
Citation | Journal: Biochem.Biophys.Res.Commun. / Year: 2005 Title: 2.0A crystal structure of human ARL5-GDP3'P, a novel member of the small GTP-binding proteins Authors: Wang, Z.X. / Shi, L. / Liu, J.F. / An, X.M. / Chang, W.R. / Liang, D.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1zj6.cif.gz | 47.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1zj6.ent.gz | 37 KB | Display | PDB format |
PDBx/mmJSON format | 1zj6.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1zj6_validation.pdf.gz | 888.5 KB | Display | wwPDB validaton report |
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Full document | 1zj6_full_validation.pdf.gz | 892 KB | Display | |
Data in XML | 1zj6_validation.xml.gz | 11.1 KB | Display | |
Data in CIF | 1zj6_validation.cif.gz | 14.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zj/1zj6 ftp://data.pdbj.org/pub/pdb/validation_reports/zj/1zj6 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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Unit cell |
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-Components
#1: Protein | Mass: 21824.084 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Plasmid: pET22b / Production host: Escherichia coli (E. coli) / Strain (production host): B834(DE3) / References: UniProt: Q9Y689 | ||||
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#2: Chemical | #3: Chemical | ChemComp-G3D / | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3 Å3/Da / Density % sol: 59.2 % |
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Crystal grow | Temperature: 288 K / Method: vapor diffusion, hanging drop / pH: 5.3 Details: 0.1M sodium citrate (pH5.3), 1.4M ammonium sulfate, 0.1% Triton X-305, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Photon Factory / Beamline: BL-6B / Wavelength: 1 Å |
Detector | Type: RIGAKU / Detector: IMAGE PLATE / Date: Nov 11, 2004 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2→500 Å / Num. all: 18304 / Num. obs: 16149 / % possible obs: 88.2 % |
Reflection shell | Resolution: 2→2.06 Å / % possible all: 91 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2→38.39 Å / σ(F): -3
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Refinement step | Cycle: LAST / Resolution: 2→38.39 Å
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