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- PDB-1z97: Human Carbonic Anhydrase III: Structural and Kinetic Study of Cat... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1z97 | ||||||
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Title | Human Carbonic Anhydrase III: Structural and Kinetic Study of Catalysis and Proton Transfer. | ||||||
![]() | Carbonic anhydrase III | ||||||
![]() | LYASE / carbonic anhydrase / proton wire / chemical rescue | ||||||
Function / homology | ![]() nickel cation binding / Reversible hydration of carbon dioxide / carbonic anhydrase / carbonate dehydratase activity / response to bacterium / one-carbon metabolic process / zinc ion binding / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Duda, D.M. / Tu, C. / Fisher, S.Z. / An, H. / Yoshioka, C. / Govindasamy, L. / Laipis, P.J. / Agbandje-McKenna, M. / Silverman, D.N. / McKenna, R. | ||||||
![]() | ![]() Title: Human Carbonic Anhydrase III: Structural and Kinetic Study of Catalysis and Proton Transfer Authors: Duda, D.M. / Tu, C. / Fisher, S.Z. / An, H. / Yoshioka, C. / Govindasamy, L. / Laipis, P.J. / Agbandje-McKenna, M. / Silverman, D.N. / McKenna, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 67.8 KB | Display | ![]() |
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PDB format | ![]() | 48.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 432 KB | Display | ![]() |
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Full document | ![]() | 439 KB | Display | |
Data in XML | ![]() | 13.6 KB | Display | |
Data in CIF | ![]() | 17.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1z93C ![]() 1fljS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 30376.203 Da / Num. of mol.: 1 / Mutation: F198L, C182S, C187S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-ZN / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.07 Å3/Da / Density % sol: 40.55 % |
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Crystal grow | Temperature: 273 K / Method: vapor diffusion, hanging drop / pH: 9.2 Details: 30% PEG 8000, 10mM Tris, pH 9.2, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Jan 9, 2002 / Details: Osmic mirror |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→20 Å / Num. obs: 13525 / % possible obs: 92.3 % / Observed criterion σ(F): 3 / Rsym value: 0.081 |
Reflection shell | Resolution: 2.1→2.17 Å / Num. unique all: 1268 / Rsym value: 0.341 / % possible all: 87.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: pdb entry 1FLJ Resolution: 2.1→20 Å / Cross valid method: THROUGHOUT / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.1→20 Å
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Refine LS restraints |
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