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- PDB-1z7n: ATP Phosphoribosyl transferase (HisZG ATP-PRTase) from Lactococcu... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1z7n | ||||||
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Title | ATP Phosphoribosyl transferase (HisZG ATP-PRTase) from Lactococcus lactis with bound PRPP substrate | ||||||
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![]() | TRANSFERASE / ATP-PRT / PRPP / histidine biosynthesis / HisZG / allosteric | ||||||
Function / homology | ![]() ATP phosphoribosyltransferase / ATP phosphoribosyltransferase activity / histidine-tRNA ligase activity / histidyl-tRNA aminoacylation / L-histidine biosynthetic process / transferase activity / ATP binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Champagne, K.S. / Sissler, M. / Larrabee, Y. / Doublie, S. / Francklyn, C.S. | ||||||
![]() | ![]() Title: Activation of the hetero-octameric ATP phosphoribosyl transferase through subunit interface rearrangement by a tRNA synthetase paralog. Authors: Champagne, K.S. / Sissler, M. / Larrabee, Y. / Doublie, S. / Francklyn, C.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 377.5 KB | Display | ![]() |
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PDB format | ![]() | 315.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.5 MB | Display | ![]() |
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Full document | ![]() | 1.6 MB | Display | |
Data in XML | ![]() | 78.4 KB | Display | |
Data in CIF | ![]() | 103 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 39848.445 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: hisZ / Plasmid: pQE30 / Production host: ![]() ![]() #2: Protein | Mass: 23708.254 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: hisG / Plasmid: pQE30 / Production host: ![]() ![]() #3: Sugar | ChemComp-PRP / #4: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.17 Å3/Da / Density % sol: 60.83 % |
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Crystal grow | Temperature: 285 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: PEG 400, Tris-HCL, MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: BRANDEIS - B1.2 / Detector: CCD / Date: Aug 2, 2001 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.921 Å / Relative weight: 1 |
Reflection | Resolution: 3.25→29 Å / Num. all: 85130 / Num. obs: 85130 / % possible obs: 98.1 % / Redundancy: 4 % / Biso Wilson estimate: 64 Å2 / Rmerge(I) obs: 0.123 / Rsym value: 0.085 / Net I/σ(I): 9.52 |
Reflection shell | Resolution: 3.25→3.45 Å / Rmerge(I) obs: 0.402 / Mean I/σ(I) obs: 2.28 / Num. unique all: 7127 / Rsym value: 0.341 / % possible all: 93.7 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.25→29 Å
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Refine LS restraints |
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