- PDB-1yyp: Crystal structure of cytomegalovirus UL44 bound to C-terminal pep... -
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Basic information
Entry
Database: PDB / ID: 1yyp
Title
Crystal structure of cytomegalovirus UL44 bound to C-terminal peptide from CMV UL54
Components
DNA polymerase
DNA polymerase processivity factor
Keywords
REPLICATION/TRANSFERASE / PROCESSIVITY FOLD (SAME FOLD AS HSV UL42 / PCNA / AND HOMOTRIMERIC SLIDING CLAMPS) / REPLICATION-TRANSFERASE COMPLEX
Function / homology
Function and homology information
bidirectional double-stranded viral DNA replication / DNA polymerase processivity factor activity / virion component / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity / DNA replication / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / virus-mediated perturbation of host defense response / nucleotide binding / host cell nucleus / DNA binding Similarity search - Function
Herpesvirus polymerase accessory protein / Herpesvirus polymerase accessory protein / Box / Proliferating Cell Nuclear Antigen / Proliferating Cell Nuclear Antigen - #10 / DNA polymerase family B signature. / DNA-directed DNA polymerase, family B, conserved site / : / DNA polymerase family B / DNA polymerase family B, exonuclease domain ...Herpesvirus polymerase accessory protein / Herpesvirus polymerase accessory protein / Box / Proliferating Cell Nuclear Antigen / Proliferating Cell Nuclear Antigen - #10 / DNA polymerase family B signature. / DNA-directed DNA polymerase, family B, conserved site / : / DNA polymerase family B / DNA polymerase family B, exonuclease domain / DNA-directed DNA polymerase, family B, exonuclease domain / DNA-directed DNA polymerase, family B, multifunctional domain / DNA polymerase type-B family / DNA-directed DNA polymerase, family B / Ribonuclease H superfamily / Ribonuclease H-like superfamily / DNA/RNA polymerase superfamily / Alpha Beta Similarity search - Domain/homology
#2: Journal: J.Virol. / Year: 2004 Title: Specific residues in the connector loop of the human cytomegalovirus DNA polymerase accessory protein UL44 are crucial for interaction with the UL54 catalytic subunit
#3: Journal: J.Virol. / Year: 2004 Title: Residues of human cytomegalovirus DNA polymerase catalytic subunit UL54 that are necessary and sufficient for interaction with the accessory protein UL44
#4: Journal: J.Virol. / Year: 1992 Title: Physical and functional interaction of human cytomegalovirus DNA polymerase and its accessory protein (ICP36) expressed in insect cells
History
Deposition
Feb 25, 2005
Deposition site: RCSB / Processing site: RCSB
Revision 1.0
Dec 27, 2005
Provider: repository / Type: Initial release
Revision 1.1
Apr 30, 2008
Group: Version format compliance
Revision 1.2
Jul 13, 2011
Group: Advisory / Refinement description / Version format compliance
SEQUENCE AUTHOR STATES THAT THE SER 205 IS INDEED CORRECT AND IT MAY REPRESENT A DIFFERENT ISOLATE ...SEQUENCE AUTHOR STATES THAT THE SER 205 IS INDEED CORRECT AND IT MAY REPRESENT A DIFFERENT ISOLATE CYTOMEGALOVIRUS, REFERRING TO ERTL PF, POWELL KL. "PHYSICAL AND FUNCTIONAL INTERACTION OF HUMAN CYTOMEGALOVIRUS DNA POLYMERASE AND ITS ACCESSORY PROTEIN (ICP36) EXPRESSED IN INSECT CELLS." J VIROL. 1992 JUL;66(7):4126-33.
Mass: 18.015 Da / Num. of mol.: 48 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.8 Å3/Da / Density % sol: 55 %
Crystal grow
Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 4.2 Details: 2 M ammonium sulfate, 100 mM phosphate-citrate, and 10 mM DTT , pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K
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