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- PDB-1yhz: Crystal structure of Arabidopsis thaliana Acetohydroxyacid syntha... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1yhz | ||||||
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Title | Crystal structure of Arabidopsis thaliana Acetohydroxyacid synthase In Complex With A Sulfonylurea Herbicide, Chlorsulfuron | ||||||
![]() | Acetolactate synthase | ||||||
![]() | TRANSFERASE / Acetohydroxyacid synthase / acetolactate synthase / herbicide / sulfonylurea / thiamin diphosphate / FAD / inhibitor / cysteine-s-dioxide / CHES / chlorsulfuron | ||||||
Function / homology | ![]() acetolactate synthase / acetolactate synthase complex / acetolactate synthase activity / valine biosynthetic process / isoleucine biosynthetic process / response to herbicide / thiamine pyrophosphate binding / chloroplast stroma / amino acid biosynthetic process / chloroplast ...acetolactate synthase / acetolactate synthase complex / acetolactate synthase activity / valine biosynthetic process / isoleucine biosynthetic process / response to herbicide / thiamine pyrophosphate binding / chloroplast stroma / amino acid biosynthetic process / chloroplast / flavin adenine dinucleotide binding / magnesium ion binding / mitochondrion Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | McCourt, J.A. / Pang, S.S. / King-Scott, J. / Guddat, L.W. / Duggleby, R.G. | ||||||
![]() | ![]() Title: Herbicide-binding sites revealed in the structure of plant acetohydroxyacid synthase Authors: McCourt, J.A. / Pang, S.S. / King-Scott, J. / Guddat, L.W. / Duggleby, R.G. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 139.2 KB | Display | ![]() |
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PDB format | ![]() | 103.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 28.8 KB | Display | |
Data in CIF | ![]() | 41.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1ybhSC ![]() 1yhyC ![]() 1yi0C ![]() 1yi1C ![]() 1z8nC C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Details | The biological unit is a tetramer |
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Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 64591.664 Da / Num. of mol.: 1 / Fragment: residues 86-667 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Non-polymers , 6 types, 349 molecules ![](data/chem/img/MG.gif)
![](data/chem/img/1CS.gif)
![](data/chem/img/P22.gif)
![](data/chem/img/FAD.gif)
![](data/chem/img/NHE.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/1CS.gif)
![](data/chem/img/P22.gif)
![](data/chem/img/FAD.gif)
![](data/chem/img/NHE.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-MG / |
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#3: Chemical | ChemComp-1CS / |
#4: Chemical | ChemComp-P22 / |
#5: Chemical | ChemComp-FAD / |
#6: Chemical | ChemComp-NHE / |
#7: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 6.6 Å3/Da / Density % sol: 80.6 % |
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, hanging drop Details: Tris, FAD, DTT, ThDP, magnesium chloride, chlorsulfuron, CHES, lithium sufate, potassium sodium tartrate , pH 9.0-9.8, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Dec 12, 2003 / Details: mirrors |
Radiation | Monochromator: GE(III) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→50 Å / Num. all: 48379 / Num. obs: 48379 / % possible obs: 99.5 % / Observed criterion σ(F): -3 / Observed criterion σ(I): 0 / Redundancy: 6.6 % / Rmerge(I) obs: 0.076 / Net I/σ(I): 12 |
Reflection shell | Resolution: 2.7→2.8 Å / Redundancy: 6.6 % / Mean I/σ(I) obs: 5.7 / Num. unique all: 4700 / % possible all: 98.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1YBH Resolution: 2.7→50 Å / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 42.775 Å2 | |||||||||||||||||||||||||
Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.7→50 Å
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LS refinement shell | Resolution: 2.7→2.8 Å
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