+Open data
-Basic information
Entry | Database: PDB / ID: 1ycr | ||||||
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Title | MDM2 BOUND TO THE TRANSACTIVATION DOMAIN OF P53 | ||||||
Components |
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Keywords | COMPLEX (ONCOGENE PROTEIN/PEPTIDE) / ANTI-ONCOGENE / DNA-BINDING / TRANSCRIPTION REGULATION / NUCLEAR PROTEIN / COMPLEX (ONCOGENE PROTEIN-PEPTIDE) / PHOSPHORYLATION / ACTIVATOR / COMPLEX (ONCOGENE PROTEIN-PEPTIDE) complex | ||||||
Function / homology | Function and homology information cellular response to vitamin B1 / response to formaldehyde / response to water-immersion restraint stress / response to ether / traversing start control point of mitotic cell cycle / negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator / negative regulation of signal transduction by p53 class mediator / fibroblast activation / atrial septum development / receptor serine/threonine kinase binding ...cellular response to vitamin B1 / response to formaldehyde / response to water-immersion restraint stress / response to ether / traversing start control point of mitotic cell cycle / negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator / negative regulation of signal transduction by p53 class mediator / fibroblast activation / atrial septum development / receptor serine/threonine kinase binding / Trafficking of AMPA receptors / positive regulation of vascular associated smooth muscle cell migration / Loss of function of TP53 in cancer due to loss of tetramerization ability / Regulation of TP53 Expression / signal transduction by p53 class mediator / negative regulation of G1 to G0 transition / negative regulation of glucose catabolic process to lactate via pyruvate / Transcriptional activation of cell cycle inhibitor p21 / regulation of intrinsic apoptotic signaling pathway by p53 class mediator / peroxisome proliferator activated receptor binding / Activation of NOXA and translocation to mitochondria / negative regulation of pentose-phosphate shunt / ATP-dependent DNA/DNA annealing activity / negative regulation of helicase activity / regulation of cell cycle G2/M phase transition / intrinsic apoptotic signaling pathway in response to hypoxia / regulation of fibroblast apoptotic process / response to iron ion / oxidative stress-induced premature senescence / oligodendrocyte apoptotic process / negative regulation of miRNA processing / positive regulation of thymocyte apoptotic process / glucose catabolic process to lactate via pyruvate / regulation of tissue remodeling / negative regulation of protein processing / positive regulation of mitochondrial membrane permeability / negative regulation of mitophagy / SUMO transferase activity / response to steroid hormone / positive regulation of programmed necrotic cell death / NEDD8 ligase activity / mRNA transcription / bone marrow development / circadian behavior / histone deacetylase regulator activity / T cell proliferation involved in immune response / regulation of mitochondrial membrane permeability involved in apoptotic process / RUNX3 regulates CDKN1A transcription / germ cell nucleus / AKT phosphorylates targets in the cytosol / regulation of DNA damage response, signal transduction by p53 class mediator / cellular response to peptide hormone stimulus / TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain / atrioventricular valve morphogenesis / TP53 Regulates Transcription of Death Receptors and Ligands / Activation of PUMA and translocation to mitochondria / negative regulation of neuroblast proliferation / DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator / ventricular septum development / endocardial cushion morphogenesis / Formation of Senescence-Associated Heterochromatin Foci (SAHF) / negative regulation of glial cell proliferation / Regulation of TP53 Activity through Association with Co-factors / positive regulation of execution phase of apoptosis / mitochondrial DNA repair / T cell lineage commitment / cellular response to alkaloid / positive regulation of muscle cell differentiation / negative regulation of DNA replication / SUMOylation of ubiquitinylation proteins / ER overload response / B cell lineage commitment / thymocyte apoptotic process / blood vessel development / positive regulation of cardiac muscle cell apoptotic process / TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain / regulation of protein catabolic process / TP53 Regulates Transcription of Caspase Activators and Caspases / entrainment of circadian clock by photoperiod / cardiac septum morphogenesis / PI5P Regulates TP53 Acetylation / Association of TriC/CCT with target proteins during biosynthesis / Constitutive Signaling by AKT1 E17K in Cancer / Zygotic genome activation (ZGA) / negative regulation of DNA damage response, signal transduction by p53 class mediator / necroptotic process / negative regulation of telomere maintenance via telomerase / positive regulation of release of cytochrome c from mitochondria / rRNA transcription / TP53 Regulates Transcription of Genes Involved in Cytochrome C Release / response to magnesium ion / TFIID-class transcription factor complex binding / protein sumoylation / mitophagy / ligase activity / SUMOylation of transcription factors / protein localization to nucleus / intrinsic apoptotic signaling pathway by p53 class mediator / neuroblast proliferation / general transcription initiation factor binding Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 2.6 Å | ||||||
Authors | Kussie, P.H. / Pavletich, N.P. | ||||||
Citation | Journal: Science / Year: 1996 Title: Structure of the MDM2 oncoprotein bound to the p53 tumor suppressor transactivation domain. Authors: Kussie, P.H. / Gorina, S. / Marechal, V. / Elenbaas, B. / Moreau, J. / Levine, A.J. / Pavletich, N.P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ycr.cif.gz | 31.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ycr.ent.gz | 20.8 KB | Display | PDB format |
PDBx/mmJSON format | 1ycr.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/yc/1ycr ftp://data.pdbj.org/pub/pdb/validation_reports/yc/1ycr | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 12535.350 Da / Num. of mol.: 1 / Fragment: RESIDUES 17 - 125 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Plasmid: PET3D / Production host: Escherichia coli (E. coli) / References: UniProt: Q00987 |
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#2: Protein/peptide | Mass: 1807.973 Da / Num. of mol.: 1 / Fragment: RESIDUES 15 - 29 Source method: isolated from a genetically manipulated source References: UniProt: P04637 |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.09 Å3/Da / Density % sol: 41.03 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 4 ℃ / pH: 6.8 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: RIGAKU / Detector: IMAGE PLATE / Date: Jul 25, 1996 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 3559 / % possible obs: 90 % / Observed criterion σ(I): 0 / Redundancy: 4.5 % / Rmerge(I) obs: 0.052 |
Reflection | *PLUS Highest resolution: 2.6 Å / Num. measured all: 16069 |
-Processing
Software |
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Refinement | Resolution: 2.6→8 Å / σ(F): 1 /
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Refinement step | Cycle: LAST / Resolution: 2.6→8 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.2 / Rfactor Rwork: 0.2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |