+Open data
-Basic information
Entry | Database: PDB / ID: 1xz2 | ||||||
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Title | wild-type hemoglobin deoxy no-salt | ||||||
Components |
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Keywords | TRANSPORT PROTEIN / hemoglobin / globin | ||||||
Function / homology | Function and homology information cellular oxidant detoxification / nitric oxide transport / hemoglobin binding / hemoglobin alpha binding / haptoglobin binding / haptoglobin-hemoglobin complex / organic acid binding / renal absorption / hemoglobin complex / oxygen transport ...cellular oxidant detoxification / nitric oxide transport / hemoglobin binding / hemoglobin alpha binding / haptoglobin binding / haptoglobin-hemoglobin complex / organic acid binding / renal absorption / hemoglobin complex / oxygen transport / Scavenging of heme from plasma / endocytic vesicle lumen / blood vessel diameter maintenance / hydrogen peroxide catabolic process / oxygen carrier activity / Late endosomal microautophagy / Heme signaling / carbon dioxide transport / response to hydrogen peroxide / Erythrocytes take up oxygen and release carbon dioxide / Erythrocytes take up carbon dioxide and release oxygen / Cytoprotection by HMOX1 / oxygen binding / regulation of blood pressure / platelet aggregation / Chaperone Mediated Autophagy / peroxidase activity / positive regulation of nitric oxide biosynthetic process / tertiary granule lumen / Factors involved in megakaryocyte development and platelet production / ficolin-1-rich granule lumen / blood microparticle / iron ion binding / heme binding / Neutrophil degranulation / extracellular space / extracellular exosome / extracellular region / membrane / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.9 Å | ||||||
Authors | Kavanaugh, J.S. / Rogers, P.H. / Arnone, A. / Hui, H.L. / Wierzba, A. / DeYoung, A. / Kwiatkowski, L.D. / Noble, R.W. / Juszczak, L.J. / Peterson, E.S. / Friedman, J.M. | ||||||
Citation | Journal: Biochemistry / Year: 2005 Title: Intersubunit interactions associated with tyr42alpha stabilize the quaternary-T tetramer but are not major quaternary constraints in deoxyhemoglobin Authors: Kavanaugh, J.S. / Rogers, P.H. / Arnone, A. / Hui, H.L. / Wierzba, A. / Deyoung, A. / Kwiatkowski, L.D. / Noble, R.W. / Juszczak, L.J. / Peterson, E.S. / Friedman, J.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1xz2.cif.gz | 127 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1xz2.ent.gz | 100.3 KB | Display | PDB format |
PDBx/mmJSON format | 1xz2.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1xz2_validation.pdf.gz | 1.7 MB | Display | wwPDB validaton report |
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Full document | 1xz2_full_validation.pdf.gz | 1.7 MB | Display | |
Data in XML | 1xz2_validation.xml.gz | 25.2 KB | Display | |
Data in CIF | 1xz2_validation.cif.gz | 34 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/xz/1xz2 ftp://data.pdbj.org/pub/pdb/validation_reports/xz/1xz2 | HTTPS FTP |
-Related structure data
Related structure data | 1xyeC 1xz4C 1hbbS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | the crystallographic asymmetric unit in this entry is an alpha2beta2 tetramer. The biological unit is an alpha2beta2 tetramer. The cystallogrophic asymmetric unit and biological unit are equivalent |
-Components
#1: Protein | Mass: 15150.353 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / Tissue: blood / References: UniProt: P01922, UniProt: P69905*PLUS #2: Protein | Mass: 15890.198 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / Tissue: blood / References: UniProt: P68871 #3: Chemical | ChemComp-HEM / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.41 Å3/Da / Density % sol: 49.02 % |
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Crystal grow | Temperature: 298 K / Method: batch / pH: 7 Details: 12% PEG 1450, 10 mg/ml Hb, pH 7.0, batch, temperature 298.0K |
-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 Å |
Detector | Type: SDMS / Detector: AREA DETECTOR / Date: Mar 7, 1994 / Details: graphite |
Radiation | Monochromator: graphite / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.93→50 Å / Num. all: 44690 / Num. obs: 44690 / % possible obs: 96.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 9 % / Rmerge(I) obs: 0.059 / Net I/σ(I): 12.4 |
Reflection shell | Resolution: 1.93→2.07 Å / Redundancy: 5.8 % / Rmerge(I) obs: 0.185 / Mean I/σ(I) obs: 2.9 / Num. unique all: 7546 / % possible all: 83.2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: pdb entry 1HBB Resolution: 1.9→10 Å / Cor.coef. Fo:Fc: 0.959 / Cor.coef. Fo:Fc free: 0.946 / SU B: 4.589 / SU ML: 0.134 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.162 / ESU R Free: 0.14 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 19.663 Å2
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Refinement step | Cycle: LAST / Resolution: 1.9→10 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.9→2.046 Å / Total num. of bins used: 7 /
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