+Open data
-Basic information
Entry | Database: PDB / ID: 1xyj | ||||||
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Title | NMR Structure of the cat prion protein | ||||||
Components | prion proteinPRNP | ||||||
Keywords | UNKNOWN FUNCTION / prion / TSE / PrP / Prnp | ||||||
Function / homology | Function and homology information : / protein homooligomerization / copper ion binding / Golgi apparatus / plasma membrane Similarity search - Function | ||||||
Biological species | Felis catus (domestic cat) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Lysek, D.A. / Schorn, C. / Nivon, L.G. / Esteve-Moya, V. / Christen, B. / Calzolai, L. / von Schroetter, C. / Fiorito, F. / Herrmann, T. / Guntert, P. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2005 Title: Prion protein NMR structures of cats, dogs, pigs, and sheep Authors: Lysek, D.A. / Schorn, C. / Nivon, L.G. / Esteve-Moya, V. / Christen, B. / Calzolai, L. / von Schroetter, C. / Fiorito, F. / Herrmann, T. / Guntert, P. / Wuthrich, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1xyj.cif.gz | 689.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1xyj.ent.gz | 598 KB | Display | PDB format |
PDBx/mmJSON format | 1xyj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/xy/1xyj ftp://data.pdbj.org/pub/pdb/validation_reports/xy/1xyj | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13192.662 Da / Num. of mol.: 1 / Fragment: residues 121-231 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Felis catus (domestic cat) / Gene: Prnp / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: Q5YCW7, UniProt: O18754*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 3D techniques. |
-Sample preparation
Details | Contents: 1mM fPrP U-15N, 13C; 10mM acetate buffer, pH 4.5 / Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 10mM / pH: 4.5 / Pressure: ambient / Temperature: 293 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 750 MHz |
-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||
NMR representative | Selection criteria: fewest violations | ||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 20 / Conformers submitted total number: 20 |