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- PDB-1xqm: Variations on the GFP chromophore scaffold: A fragmented 5-member... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1xqm | ||||||
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Title | Variations on the GFP chromophore scaffold: A fragmented 5-membered heterocycle revealed in the 2.1A crystal structure of a non-fluorescent chromoprotein | ||||||
![]() | kindling fluorescent protein | ||||||
![]() | LUMINESCENT PROTEIN | ||||||
Function / homology | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Wilmann, P.G. / Petersen, J. / Devenish, R.J. / Prescott, M. / Rossjohn, J. | ||||||
![]() | ![]() Title: Variations on the GFP chromophore: A polypeptide fragmentation within the chromophore revealed in the 2.1-A crystal structure of a nonfluorescent chromoprotein from Anemonia sulcata Authors: Wilmann, P.G. / Petersen, J. / Devenish, R.J. / Prescott, M. / Rossjohn, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 55.7 KB | Display | ![]() |
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PDB format | ![]() | 44.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 382.5 KB | Display | ![]() |
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Full document | ![]() | 385.1 KB | Display | |
Data in XML | ![]() | 6.9 KB | Display | |
Data in CIF | ![]() | 10.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 25838.486 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: synthetic construct, It is based on the sequence from Anemonia sulcata. References: GenBank: 28629493, UniProt: Q9GZ28*PLUS |
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#2: Chemical | ChemComp-ACY / |
#3: Water | ChemComp-HOH / |
Sequence details | MET A 63, TYR A 64 AND GLY A 65 ARE MODIFIED TO MAKE CHROMOPHOR |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.9 Å3/Da / Density % sol: 68.3 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 5.7 Details: PEG3350, Ammonium Acetate, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Nov 20, 2003 |
Radiation | Monochromator: osmic mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→50 Å / Num. all: 21669 / Num. obs: 21515 / % possible obs: 98.6 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 |
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Processing
Software | Name: CNS / Classification: refinement | |||||||||||||||
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 2.1→50 Å
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