+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1xda | ||||||
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タイトル | STRUCTURE OF INSULIN | ||||||
要素 | (FATTY ACID ACYLATED ...) x 2 | ||||||
キーワード | HORMONE / METABOLIC ROLE / CHEMICAL ACTIVITY / INSULIN ALBUMIN / FATTY ACID / GLUCOSE METABOLISM / DIABETES | ||||||
機能・相同性 | 機能・相同性情報 negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / regulation of cellular amino acid metabolic process / positive regulation of nitric oxide mediated signal transduction / negative regulation of fatty acid metabolic process / negative regulation of feeding behavior / Signaling by Insulin receptor / IRS activation / Insulin processing / regulation of protein secretion ...negative regulation of NAD(P)H oxidase activity / negative regulation of glycogen catabolic process / regulation of cellular amino acid metabolic process / positive regulation of nitric oxide mediated signal transduction / negative regulation of fatty acid metabolic process / negative regulation of feeding behavior / Signaling by Insulin receptor / IRS activation / Insulin processing / regulation of protein secretion / positive regulation of respiratory burst / positive regulation of peptide hormone secretion / Regulation of gene expression in beta cells / negative regulation of acute inflammatory response / alpha-beta T cell activation / negative regulation of respiratory burst involved in inflammatory response / positive regulation of dendritic spine maintenance / positive regulation of glycogen biosynthetic process / Synthesis, secretion, and deacylation of Ghrelin / negative regulation of protein secretion / regulation of protein localization to plasma membrane / fatty acid homeostasis / Signal attenuation / negative regulation of lipid catabolic process / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / negative regulation of gluconeogenesis / COPI-mediated anterograde transport / positive regulation of lipid biosynthetic process / negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway / negative regulation of reactive oxygen species biosynthetic process / positive regulation of insulin receptor signaling pathway / nitric oxide-cGMP-mediated signaling / transport vesicle / positive regulation of protein autophosphorylation / Insulin receptor recycling / neuron projection maintenance / NPAS4 regulates expression of target genes / positive regulation of protein metabolic process / positive regulation of brown fat cell differentiation / positive regulation of glycolytic process / activation of protein kinase B activity / endoplasmic reticulum-Golgi intermediate compartment membrane / positive regulation of mitotic nuclear division / Insulin receptor signalling cascade / positive regulation of nitric-oxide synthase activity / positive regulation of cytokine production / positive regulation of long-term synaptic potentiation / acute-phase response / Regulation of insulin secretion / endosome lumen / positive regulation of protein secretion / positive regulation of glucose import / negative regulation of proteolysis / positive regulation of cell differentiation / regulation of transmembrane transporter activity / insulin-like growth factor receptor binding / wound healing / insulin receptor binding / regulation of synaptic plasticity / negative regulation of protein catabolic process / hormone activity / cognition / positive regulation of neuron projection development / positive regulation of protein localization to nucleus / Golgi lumen / vasodilation / glucose metabolic process / regulation of protein localization / insulin receptor signaling pathway / cell-cell signaling / glucose homeostasis / positive regulation of NF-kappaB transcription factor activity / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / positive regulation of cell growth / secretory granule lumen / protease binding / positive regulation of MAPK cascade / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of cell migration / G protein-coupled receptor signaling pathway / Amyloid fiber formation / endoplasmic reticulum lumen / Golgi membrane / negative regulation of gene expression / positive regulation of cell population proliferation / positive regulation of gene expression / regulation of DNA-templated transcription / extracellular space / extracellular region / identical protein binding 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | X線回折 / シンクロトロン / 分子置換 / 解像度: 1.8 Å | ||||||
データ登録者 | Whittingham, J.L. / Havelund, S. / Jonassen, I. | ||||||
引用 | ジャーナル: Biochemistry / 年: 1997 タイトル: Crystal structure of a prolonged-acting insulin with albumin-binding properties. 著者: Whittingham, J.L. / Havelund, S. / Jonassen, I. #1: ジャーナル: Diabetologia / 年: 1996 タイトル: Soluble, Fatty Acid Acylated Insulins Bind to Albumin and Show Protracted Action in Pigs 著者: Markussen, J. / Havelund, S. / Kurtzhals, P. / Andersen, A.S. / Halstrom, J. / Hasselager, E. / Larsen, U.D. / Ribel, U. / Schaffer, L. / Vad, K. / Jonassen, I. #2: ジャーナル: Biopolymers / 年: 1992 タイトル: The Structure of a Rhombohedral R6 Insulin Hexamer that Binds Phenol 著者: Smith, G.D. / Dodson, G.G. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1xda.cif.gz | 60.3 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1xda.ent.gz | 45.2 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1xda.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 1xda_validation.pdf.gz | 434.8 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 1xda_full_validation.pdf.gz | 442.1 KB | 表示 | |
XML形式データ | 1xda_validation.xml.gz | 7.4 KB | 表示 | |
CIF形式データ | 1xda_validation.cif.gz | 11.3 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/xd/1xda ftp://data.pdbj.org/pub/pdb/validation_reports/xd/1xda | HTTPS FTP |
-関連構造データ
-リンク
-集合体
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単位格子 |
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Components on special symmetry positions |
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-要素
-FATTY ACID ACYLATED ... , 2種, 8分子 ACEGBDFH
#1: タンパク質・ペプチド | 分子量: 2383.698 Da / 分子数: 4 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 発現宿主: Saccharomyces cerevisiae (パン酵母) / 参照: UniProt: P01308 #2: タンパク質・ペプチド | 分子量: 3332.849 Da / 分子数: 4 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 発現宿主: Saccharomyces cerevisiae (パン酵母) / 参照: UniProt: P01308 |
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-非ポリマー , 5種, 170分子
#3: 化合物 | ChemComp-IPH / #4: 化合物 | ChemComp-ZN / #5: 化合物 | ChemComp-CL / #6: 化合物 | ChemComp-MYR / #7: 水 | ChemComp-HOH / | |
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-詳細
非ポリマーの詳細 | AT THE END OF CHAINS B, D, F, AND H, ATOM C1 OF A FATTY ACID (RESIDUE 30) IS COVALENTLY LINKED TO ...AT THE END OF CHAINS B, D, F, AND H, ATOM C1 OF A FATTY ACID (RESIDUE 30) IS COVALENTLY |
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-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
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-試料調製
結晶 | マシュー密度: 2.07 Å3/Da / 溶媒含有率: 40 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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結晶化 | pH: 8.2 詳細: HANGING DROP, 0.1M TRI-SODIUM CITRATE, 6% (W/V) TRIS, 0.02% (W/V) ZINC ACETATE, PH 8.2. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
結晶化 | *PLUS 手法: 蒸気拡散法, ハンギングドロップ法 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 120 K |
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放射光源 | 由来: シンクロトロン / サイト: EMBL/DESY, HAMBURG / ビームライン: X31 / 波長: 0.93 |
検出器 | タイプ: MARRESEARCH / 検出器: IMAGE PLATE / 日付: 1996年6月1日 |
放射 | 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 0.93 Å / 相対比: 1 |
反射 | 解像度: 1.8→15 Å / Num. obs: 16624 / % possible obs: 98 % / 冗長度: 2.4 % / Biso Wilson estimate: 17.3 Å2 / Rmerge(I) obs: 0.055 |
反射 シェル | 解像度: 1.8→1.83 Å / Rmerge(I) obs: 0.278 / % possible all: 73 |
反射 | *PLUS Num. measured all: 40024 |
反射 シェル | *PLUS % possible obs: 72.7 % |
-解析
ソフトウェア |
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精密化 | 構造決定の手法: 分子置換 開始モデル: 4-IODOPHENOL INSULIN DIMER 解像度: 1.8→15 Å / σ(F): 0 /
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原子変位パラメータ | Biso mean: 19.4 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 1.8→15 Å
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拘束条件 |
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