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Yorodumi- PDB-1wy6: Crystal Structure of Hypothetical Protein [ST1625p] from Hyperthe... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1wy6 | |||||||||
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Title | Crystal Structure of Hypothetical Protein [ST1625p] from Hyperthermophilic Archaeon Sulfolobus tokodaii | |||||||||
Components | hypothetical protein ST1625Hypothesis | |||||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / hypothetical protein / helical repeat protein | |||||||||
Function / homology | Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat - #350 / Protein of unknown function DUF1955 / Hypothetical protein ST1625 / Domain of unknown function (DUF1955) / Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat / Alpha Horseshoe / Mainly Alpha / Uncharacterized protein Function and homology information | |||||||||
Biological species | Sulfolobus tokodaii (archaea) | |||||||||
Method | X-RAY DIFFRACTION / MIRAS / Resolution: 2.2 Å | |||||||||
Authors | Yoneda, K. / Sakuraba, H. / Tsuge, H. / Katunuma, N. / Kuramitsu, S. / Kawabata, T. / Ohshima, T. | |||||||||
Citation | Journal: Acta Crystallogr.,Sect.F / Year: 2005 Title: The first crystal structure of an archaeal helical repeat protein. Authors: Yoneda, K. / Sakuraba, H. / Tsuge, H. / Katunuma, N. / Kuramitsu, S. / Kawabata, T. / Ohshima, T. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1wy6.cif.gz | 39.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1wy6.ent.gz | 31.1 KB | Display | PDB format |
PDBx/mmJSON format | 1wy6.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wy/1wy6 ftp://data.pdbj.org/pub/pdb/validation_reports/wy/1wy6 | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 19413.566 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sulfolobus tokodaii (archaea) / Plasmid: pET11a / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta-gamiTM (DE3) / References: UniProt: Q970G9 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 2 |
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-Sample preparation
Crystal | Density Matthews: 1.9 Å3/Da / Density % sol: 35.9 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 4.2 Details: 20% PEG 8000, 100mM phosphate/citrate, 100mM NaCl, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU / Wavelength: 1.5418 Å |
Detector | Type: RIGAKU RAXIS VII / Detector: IMAGE PLATE / Date: Oct 24, 2003 |
Radiation | Monochromator: SI / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→500 Å / Num. obs: 127292 / Biso Wilson estimate: 15.5 Å2 |
-Processing
Software |
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Refinement | Method to determine structure: MIRAS / Resolution: 2.2→50 Å / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 2.2→50 Å
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