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Yorodumi- PDB-1wwf: NMR Structure Determined for MLV NC Complex with RNA Sequence CCUCCGU -
+Open data
-Basic information
Entry | Database: PDB / ID: 1wwf | ||||||
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Title | NMR Structure Determined for MLV NC Complex with RNA Sequence CCUCCGU | ||||||
Components |
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Keywords | Viral protein/RNA / Hydrophobic Guanosine Binding pocket / Viral protein-RNA COMPLEX | ||||||
Function / homology | Function and homology information host cell uropod / host cell late endosome membrane / viral budding via host ESCRT complex / host multivesicular body / viral nucleocapsid / structural constituent of virion / host cell plasma membrane / RNA binding / zinc ion binding / membrane Similarity search - Function | ||||||
Biological species | Moloney murine leukemia virus | ||||||
Method | SOLUTION NMR / distance geometry | ||||||
Authors | Dey, A. / York, D. / Smalls-Mantey, A. / Summers, M.F. | ||||||
Citation | Journal: Biochemistry / Year: 2005 Title: Composition and sequence-dependent binding of RNA to the nucleocapsid protein of Moloney murine leukemia virus(,) Authors: Dey, A. / York, D. / Smalls-Mantey, A. / Summers, M.F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1wwf.cif.gz | 444.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1wwf.ent.gz | 368.1 KB | Display | PDB format |
PDBx/mmJSON format | 1wwf.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1wwf_validation.pdf.gz | 379.4 KB | Display | wwPDB validaton report |
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Full document | 1wwf_full_validation.pdf.gz | 599.7 KB | Display | |
Data in XML | 1wwf_validation.xml.gz | 27.7 KB | Display | |
Data in CIF | 1wwf_validation.cif.gz | 43.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ww/1wwf ftp://data.pdbj.org/pub/pdb/validation_reports/ww/1wwf | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: RNA chain | Mass: 2133.307 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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#2: Protein | Mass: 6377.248 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Moloney murine leukemia virus / Genus: Gammaretrovirus / Species: Murine leukemia virus / Plasmid: pGEX-6P-1 / Production host: Escherichia coli (E. coli) / References: UniProt: P03332 |
#3: Chemical | ChemComp-ZN / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D homonuclear techniques |
-Sample preparation
Details |
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Sample conditions | Ionic strength: 10mM Tris-HCl, pH 7.0, 10mM NaCl, 0.1mM ZnCl2 pH: 7.0 / Pressure: 1 atm / Temperature: 288 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: distance geometry / Software ordinal: 1 Details: The structures are based on a total of following restraints for: RNA:35 intraresidue restraints,37 intermolecular NOE restraints and 12 inter-molecular H-Bond restraints; NC Protein: 22 ...Details: The structures are based on a total of following restraints for: RNA:35 intraresidue restraints,37 intermolecular NOE restraints and 12 inter-molecular H-Bond restraints; NC Protein: 22 intraresidue restraints and 40 H-Bond restraints | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 40 / Conformers submitted total number: 20 |