Journal: J.Biol.Chem. / Year: 2001 Title: The solution structure of the complex formed between alpha-bungarotoxin and an 18-mer cognate peptide derived from the alpha 1 subunit of the nicotinic acetylcholine receptor from Torpedo californica. Authors: Zeng, H. / Moise, L. / Grant, M.A. / Hawrot, E.
Mass: 2424.732 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Torpedo californica (Pacific electric ray) Plasmid: PET-31B(+) / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P02710
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
3D 15N-separated TOCSY
1
2
1
3D HNHA
1
3
1
3D 15N-separated NOESY
1
4
1
2D TOCSY
1
5
1
2D NOESY
1
6
1
2D 1H-15N HSQC
NMR details
Text: The chirality error is in THR 6 CB, OG1; ILE 11 CB, CG2; and THR 196 CB, OG1. There is no chirality error if MOLMOL is used to view the structure.
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Sample preparation
Details
Contents: 2.1 mM alpha-Bungarotoxin/alpha18mer complex, alpha18mer is 15N labeled, 50 mM perdeuterated potassium acetate buffer (pH 4.0) with 5% D2O and 0.05% sodium azide Solvent system: 95% H2O/5% D2O
Sample conditions
pH: 4.0 / Pressure: ambient / Temperature: 308 K
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NMR measurement
NMR spectrometer
Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz
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