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Yorodumi- PDB-1wov: Crystal structure of heme oxygenase-2 from Synechocystis sp. PCC ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1wov | ||||||
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Title | Crystal structure of heme oxygenase-2 from Synechocystis sp. PCC 6803 in complex with heme | ||||||
Components | Heme oxygenase 2 | ||||||
Keywords | OXIDOREDUCTASE / HOMO DIMER | ||||||
Function / homology | Function and homology information heme oxygenase (biliverdin-producing) / heme oxidation / heme oxygenase (decyclizing) activity / heme catabolic process / photosynthesis / response to oxidative stress / heme binding / metal ion binding Similarity search - Function | ||||||
Biological species | Synechocystis sp. PCC 6803 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.75 Å | ||||||
Authors | Sugishima, M. / Hagiwara, Y. / Zhang, X. / Yoshida, T. / Migita, C.T. / Fukuyama, K. | ||||||
Citation | Journal: Biochemistry / Year: 2005 Title: Crystal structure of dimeric heme oxygenase-2 from Synechocystis sp. PCC 6803 in complex with heme. Authors: Sugishima, M. / Hagiwara, Y. / Zhang, X. / Yoshida, T. / Migita, C.T. / Fukuyama, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1wov.cif.gz | 128.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1wov.ent.gz | 99.4 KB | Display | PDB format |
PDBx/mmJSON format | 1wov.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1wov_validation.pdf.gz | 1.1 MB | Display | wwPDB validaton report |
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Full document | 1wov_full_validation.pdf.gz | 1.1 MB | Display | |
Data in XML | 1wov_validation.xml.gz | 29.2 KB | Display | |
Data in CIF | 1wov_validation.cif.gz | 43.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wo/1wov ftp://data.pdbj.org/pub/pdb/validation_reports/wo/1wov | HTTPS FTP |
-Related structure data
Related structure data | 1wowC 1woxC 1dveS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Details | The biological assembly is a dimer in the asymmetric unit. |
-Components
#1: Protein | Mass: 28572.312 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Synechocystis sp. PCC 6803 (bacteria) / Plasmid: pMW-A / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) References: UniProt: P74133, heme oxygenase (biliverdin-producing) #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.6 Å3/Da / Density % sol: 52.8 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: pottasium sodium tartrate, pottasium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL41XU / Wavelength: 1.063 Å |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Mar 12, 2004 |
Radiation | Monochromator: Si(111) Double monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.063 Å / Relative weight: 1 |
Reflection | Resolution: 1.75→20 Å / Num. all: 53138 / Num. obs: 53138 / % possible obs: 89.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 |
Reflection shell | Resolution: 1.75→1.84 Å / % possible all: 93 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1DVE Resolution: 1.75→19.76 Å / Cor.coef. Fo:Fc: 0.945 / Cor.coef. Fo:Fc free: 0.913 / SU B: 2.76 / SU ML: 0.09 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.137 / ESU R Free: 0.143 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 19.087 Å2
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Refinement step | Cycle: LAST / Resolution: 1.75→19.76 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.75→1.795 Å / Total num. of bins used: 20 /
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