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Open data
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Basic information
Entry | Database: PDB / ID: 1whi | ||||||
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Title | RIBOSOMAL PROTEIN L14 | ||||||
![]() | RIBOSOMAL PROTEIN L14 | ||||||
![]() | RIBOSOMAL PROTEIN / RRNA-BINDING | ||||||
Function / homology | ![]() large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / structural constituent of ribosome / translation Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Davies, C. / White, S.W. / Ramakrishnan, V. | ||||||
![]() | ![]() Title: The crystal structure of ribosomal protein L14 reveals an important organizational component of the translational apparatus. Authors: Davies, C. / White, S.W. / Ramakrishnan, V. #1: ![]() Title: Crystallization and Preliminary X-Ray Diffraction of Bacterial Ribosomal Protein L14 Authors: Davies, C. / Gerchman, S.E. / Kycia, J.H. / Mcgee, K. / Ramakrishnan, V. / White, S.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 36.2 KB | Display | ![]() |
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PDB format | ![]() | 25.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 13369.613 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Description: T7 EXPRESSION SYSTEM INDUCIBLE BY ISOPROPYL-BETA-D-THIOGALACTOPYRANOSIDE (IPTG) Cell line: BL21 / Gene: BACILLUS STEAROTHERMOPHILUS / Plasmid: PET13 Gene (production host): BACILLUS STEAROTHERMOPHILUS (ACCESSION P04450) Production host: ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.98 Å3/Da / Density % sol: 38.2 % |
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Crystal grow | pH: 7 / Details: pH 7.0 |
Crystal grow | *PLUS Temperature: 295 K / Method: vapor diffusion, hanging dropDetails: Davies, C., (1994) Acta Crystallogr.,Sect.D, 50, 790. |
Components of the solutions | *PLUS Conc.: 1.0-1.5 M / Common name: sodium citrate |
-Data collection
Diffraction | Mean temperature: 295 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jul 12, 1994 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→14.6 Å / Num. obs: 15965 / % possible obs: 94.5 % / Observed criterion σ(I): 0 / Redundancy: 2.44 % / Rmerge(I) obs: 0.042 |
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Processing
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Refinement | Resolution: 1.5→8 Å / σ(F): 0
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Displacement parameters | Biso mean: 13.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.5→8 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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