[English] 日本語
Yorodumi- PDB-1wgw: Solution Structure of the N-terminal Domain of Mouse Putative Sig... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1wgw | ||||||
---|---|---|---|---|---|---|---|
Title | Solution Structure of the N-terminal Domain of Mouse Putative Signal Recognition Particle 54 (SRP54) | ||||||
Components | 'Signal Recognition Particle 54 | ||||||
Keywords | SIGNALING PROTEIN / Signal recognition particle 54 (SRP54) / four helical bundle / structural genomics / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information SRP-dependent cotranslational protein targeting to membrane / signal recognition particle, endoplasmic reticulum targeting / granulocyte differentiation / endoplasmic reticulum signal peptide binding / signal-recognition-particle GTPase / SRP-dependent cotranslational protein targeting to membrane / 7S RNA binding / exocrine pancreas development / SRP-dependent cotranslational protein targeting to membrane, translocation / ribonucleoprotein complex binding ...SRP-dependent cotranslational protein targeting to membrane / signal recognition particle, endoplasmic reticulum targeting / granulocyte differentiation / endoplasmic reticulum signal peptide binding / signal-recognition-particle GTPase / SRP-dependent cotranslational protein targeting to membrane / 7S RNA binding / exocrine pancreas development / SRP-dependent cotranslational protein targeting to membrane, translocation / ribonucleoprotein complex binding / neutrophil chemotaxis / nuclear speck / GTPase activity / GTP binding / endoplasmic reticulum / ATP hydrolysis activity / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Li, H. / Tomizawa, T. / Koshiba, S. / Inoue, M. / Kigawa, T. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: To be Published Title: Solution Structure of the N-terminal Domain of Mouse Putative Signal Recognition Particle 54 (SRP54) Authors: Li, H. / Tomizawa, T. / Koshiba, S. / Inoue, M. / Kigawa, T. / Yokoyama, S. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1wgw.cif.gz | 590.5 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1wgw.ent.gz | 495.5 KB | Display | PDB format |
PDBx/mmJSON format | 1wgw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1wgw_validation.pdf.gz | 344.3 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 1wgw_full_validation.pdf.gz | 512.3 KB | Display | |
Data in XML | 1wgw_validation.xml.gz | 39.8 KB | Display | |
Data in CIF | 1wgw_validation.cif.gz | 59.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wg/1wgw ftp://data.pdbj.org/pub/pdb/validation_reports/wg/1wgw | HTTPS FTP |
-Related structure data
Similar structure data | |
---|---|
Other databases |
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | ' Mass: 10616.303 Da / Num. of mol.: 1 / Fragment: N-terminal domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Description: Cell-free protein synthesis / Gene: RIKEN cDNA 2410071O21 / Plasmid: P030908-15 / References: UniProt: P14576 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details | Contents: 1.04mM Protein U-13C, 15N; 20mM Phosphate buffer Na (pH6.0); 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 10% D2O Solvent system: 90% H2O/10% D2O |
---|---|
Sample conditions | Ionic strength: 120mM / pH: 6.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
---|---|
Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 800 MHz |
-Processing
NMR software |
| ||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations, target function Conformers calculated total number: 100 / Conformers submitted total number: 20 |