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- PDB-1w9s: Structure of a beta-1,3-glucan binding CBM6 from Bacillus halodurans -
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Open data
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Basic information
Entry | Database: PDB / ID: 1w9s | ||||||
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Title | Structure of a beta-1,3-glucan binding CBM6 from Bacillus halodurans | ||||||
![]() | BH0236 PROTEIN | ||||||
![]() | CARBOHYDRATE-BINDING MODULE / LECTIN / BETA-GLUCAN / CARBOHYDRATE BINDING / GLYCOSIDE HYDROLASE | ||||||
Function / homology | ![]() : / endo-1,3(4)-beta-glucanase activity / glucan endo-1,3-beta-D-glucosidase / glucan endo-1,3-beta-D-glucosidase activity / polysaccharide catabolic process / cell wall organization / carbohydrate binding / cell surface / extracellular region Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Boraston, A.B. / van Bueren, A.L. | ||||||
![]() | ![]() Title: Family 6 Carbohydrate Binding Modules Recognize the Non-Reducing End of Beta-1,3-Linked Glucans by Presenting a Unique Ligand Binding Surface Authors: Van Bueren, A.L. / Moreland, C. / Gilbert, H.J. / Boraston, A.B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 133.7 KB | Display | ![]() |
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PDB format | ![]() | 104 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 435.9 KB | Display | ![]() |
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Full document | ![]() | 436.3 KB | Display | |
Data in XML | ![]() | 16.1 KB | Display | |
Data in CIF | ![]() | 23.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1w9tC ![]() 1w9wC ![]() 1gmmS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 15673.985 Da / Num. of mol.: 2 / Fragment: CBM, RESIDUES 790-925 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-NA / #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.04 Å3/Da / Density % sol: 39.63 % |
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-Data collection
Diffraction | Mean temperature: 113 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.59→20 Å / Num. obs: 28471 / % possible obs: 90.1 % / Observed criterion σ(I): 2 / Redundancy: 3.7 % / Rmerge(I) obs: 0.03 / Net I/σ(I): 24.9 |
Reflection shell | Resolution: 1.59→1.65 Å / Redundancy: 3.2 % / Rmerge(I) obs: 0.17 / Mean I/σ(I) obs: 5.7 / % possible all: 68.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1GMM Resolution: 1.59→20 Å / Cor.coef. Fo:Fc: 0.978 / Cor.coef. Fo:Fc free: 0.96 / SU B: 2.93 / SU ML: 0.048 / Cross valid method: THROUGHOUT / ESU R: 0.127 / ESU R Free: 0.082 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 13.68 Å2
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Refinement step | Cycle: LAST / Resolution: 1.59→20 Å
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Refine LS restraints |
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