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Yorodumi- PDB-1vry: Second and Third Transmembrane Domains of the Alpha-1 Subunit of ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1vry | |||||||||
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Title | Second and Third Transmembrane Domains of the Alpha-1 Subunit of Human Glycine Receptor | |||||||||
Components | Glycine receptor alpha-1 chain | |||||||||
Keywords | MEMBRANE PROTEIN / GLYCINE RECEPTOR / SECOND TRANSMEMBRANE DOMAIN / THIRD TRANSMEMBRANE DOMAIN | |||||||||
Function / homology | Function and homology information taurine binding / response to alcohol / negative regulation of transmission of nerve impulse / Neurotransmitter receptors and postsynaptic signal transmission / acrosome reaction / positive regulation of acrosome reaction / neuromuscular process controlling posture / inhibitory synapse / righting reflex / regulation of respiratory gaseous exchange by nervous system process ...taurine binding / response to alcohol / negative regulation of transmission of nerve impulse / Neurotransmitter receptors and postsynaptic signal transmission / acrosome reaction / positive regulation of acrosome reaction / neuromuscular process controlling posture / inhibitory synapse / righting reflex / regulation of respiratory gaseous exchange by nervous system process / extracellularly glycine-gated chloride channel activity / synaptic transmission, glycinergic / glycinergic synapse / inhibitory postsynaptic potential / cellular response to ethanol / adult walking behavior / chloride transport / cellular response to zinc ion / glycine binding / startle response / chloride channel complex / neuronal action potential / neuropeptide signaling pathway / response to amino acid / monoatomic ion transport / chloride transmembrane transport / ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential / regulation of membrane potential / visual perception / transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential / muscle contraction / cellular response to amino acid stimulus / transmembrane signaling receptor activity / perikaryon / postsynaptic membrane / neuron projection / external side of plasma membrane / intracellular membrane-bounded organelle / neuronal cell body / dendrite / synapse / zinc ion binding / membrane / identical protein binding / plasma membrane Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | SOLUTION NMR / simulated annealing | |||||||||
Authors | Ma, D. / Liu, Z. / Li, L. / Tang, P. / Xu, Y. | |||||||||
Citation | Journal: Biochemistry / Year: 2005 Title: Structure and Dynamics of the Second and Third Transmembrane Domains of Human Glycine Receptor. Authors: Ma, D. / Liu, Z. / Li, L. / Tang, P. / Xu, Y. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1vry.cif.gz | 407.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1vry.ent.gz | 342.9 KB | Display | PDB format |
PDBx/mmJSON format | 1vry.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/vr/1vry ftp://data.pdbj.org/pub/pdb/validation_reports/vr/1vry | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 8530.070 Da / Num. of mol.: 1 / Fragment: SECOND AND THIRD TRANSMEMBRANE DOMAINS / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: GLRA1 / Plasmid: PLYSS / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: P23415 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: THE STRUCTURE WAS DETERMINED USING STANDARD 15N FILTERED NOESY SPECTROSCOPY AND H/D EXCHANGE EXPERIMENTS TOGETHER WITH HA AND CA CHEMICAL SHIFT INDEXES FOR IDENTIFICATION OF INTRAHELICAL HYDROGEN BONDING. |
-Sample preparation
Details | Contents: 1MM TM23 U-15N, TRIFLUOROETHANOL-D2; 1MM TM23 U-15N,13C, TRIFLUOROETHANOL-D2 |
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Sample conditions | pH: 7.0 / Pressure: 1 atm / Temperature: 303 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: TARGET FUNCTION,STRUCTURES WITH THE LOWEST ENERGY, STRUCTURES WITH THE LEAST RESTRAINT VIOLATIONS Conformers calculated total number: 50 / Conformers submitted total number: 20 |