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- PDB-1z0p: Crystal structure of the Protein of Unknown Function SPY1572 from... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1z0p | ||||||
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Title | Crystal structure of the Protein of Unknown Function SPY1572 from Streptococcus pyogenes | ||||||
![]() | hypothetical protein SPy1572 | ||||||
![]() | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / Streptococcus pyogenes / PSI / Protein Structure Initiative / Midwest Center for Structural Genomics / MCSG | ||||||
Function / homology | Protein of unknown function DUF1912 / SPy1572-like superfamily / Domain of unknown function (DUF1912) / Methane Monooxygenase Hydroxylase; Chain G, domain 1 - #90 / Methane Monooxygenase Hydroxylase; Chain G, domain 1 / Up-down Bundle / Mainly Alpha / Uncharacterized protein![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zhang, R. / Lezondra, L. / Clancy, S. / Collart, F. / Joachimiak, A. / Midwest Center for Structural Genomics (MCSG) | ||||||
![]() | ![]() Title: The 1.7A Crystal structure of the hypothetical protein SPy1572 from Streptococcus pyogenes Authors: Zhang, R. / Lezondra, L. / Clancy, S. / Collart, F. / Joachimiak, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 28 KB | Display | ![]() |
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PDB format | ![]() | 18.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 425.4 KB | Display | ![]() |
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Full document | ![]() | 427.2 KB | Display | |
Data in XML | ![]() | 6 KB | Display | |
Data in CIF | ![]() | 7.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Details | This protein existed as dimer.The second part of the biological assembly is generated by the axis: -x+y, -x, z |
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Components
#1: Protein | Mass: 9947.979 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.885 Å3/Da / Density % sol: 32.23 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 4.5 Details: 20% butanediol, 0.1M acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Dec 4, 2004 / Details: mirrors |
Radiation | Monochromator: Si 111 channel / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→40 Å / Num. obs: 16058 / % possible obs: 99.7 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 10.1 % / Biso Wilson estimate: 27 Å2 / Rmerge(I) obs: 0.067 / Net I/σ(I): 41.88 |
Reflection shell | Resolution: 1.7→1.76 Å / Redundancy: 8.2 % / Rmerge(I) obs: 0.596 / Mean I/σ(I) obs: 2.83 / Num. unique all: 828 / % possible all: 99.6 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 81.5796 Å2 / ksol: 0.382325 e/Å3 | |||||||||||||||||||||||||
Displacement parameters | Biso mean: 32.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.7→25.69 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.7→1.81 Å / Rfactor Rfree error: 0.031 / Total num. of bins used: 6
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Xplor file |
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