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Yorodumi- PDB-1v5i: Crystal structure of serine protease inhibitor POIA1 in complex w... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1v5i | ||||||
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Title | Crystal structure of serine protease inhibitor POIA1 in complex with subtilisin BPN' | ||||||
Components |
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Keywords | hydrolase/PROTEIN BINDING / protease-inhibitor complex / POIA1 / subtilisin / hydrolase-PROTEIN BINDING COMPLEX | ||||||
Function / homology | Function and homology information subtilisin / sporulation resulting in formation of a cellular spore / fibrinolysis / serine-type endopeptidase inhibitor activity / serine-type endopeptidase activity / proteolysis / extracellular space / metal ion binding Similarity search - Function | ||||||
Biological species | Bacillus amyloliquefaciens (bacteria) Pleurotus ostreatus (oyster mushroom) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.5 Å | ||||||
Authors | Lee, W.C. / Kikkawa, M. / Kojima, S. / Miura, K. / Tanokura, M. | ||||||
Citation | Journal: To be Published Title: Crystal structure of serine protease inhibitor POIA1 in complex with subtilisin BPN' Authors: Lee, W.C. / Kikkawa, M. / Kojima, S. / Miura, K. / Tanokura, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1v5i.cif.gz | 88.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1v5i.ent.gz | 65.8 KB | Display | PDB format |
PDBx/mmJSON format | 1v5i.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/v5/1v5i ftp://data.pdbj.org/pub/pdb/validation_reports/v5/1v5i | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein , 2 types, 2 molecules AB
#1: Protein | Mass: 27600.588 Da / Num. of mol.: 1 / Mutation: S221C Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus amyloliquefaciens (bacteria) / Production host: Escherichia coli (E. coli) / References: UniProt: P00782, subtilisin |
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#2: Protein | Mass: 8355.268 Da / Num. of mol.: 1 / Mutation: G71H, I72V, V73A, T74H, T75A, Q76Y Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pleurotus ostreatus (oyster mushroom) / Gene: POIA1 / Production host: Escherichia coli (E. coli) / References: UniProt: Q7M4T6 |
-Non-polymers , 4 types, 424 molecules
#3: Chemical | #4: Chemical | ChemComp-CA / | #5: Chemical | ChemComp-GOL / #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3.57 Å3/Da / Density % sol: 65.32 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: HEPES, lithium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL41XU / Wavelength: 0.8 Å |
Detector | Type: MARRESEARCH / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8 Å / Relative weight: 1 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.5→35.9 Å
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Refinement step | Cycle: LAST / Resolution: 1.5→35.9 Å
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