MONA/GADS SH3C IS A MONOMER IN SOLUTION, PRESENT IN THISCRYSTAL IN COMPLEX WITH AN HPK1 DERIVED PEPTIDE AND HENCEIN A HETERODIMERIC STATE.
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Components
#1: Protein
GRB2-RELATEDADAPTORPROTEIN2 / GADS PROTEIN / GROWTH FACTOR RECEPTOR BINDING PROTEIN / GRB-2-LIKE PROTEIN / GRB2L / HEMATOPOIETIC ...GADS PROTEIN / GROWTH FACTOR RECEPTOR BINDING PROTEIN / GRB-2-LIKE PROTEIN / GRB2L / HEMATOPOIETIC CELL-ASSOCIATED ADAPTOR PROTEIN GRPL / GRB-2-RELATED MONOCYTIC ADAPTER PROTEIN / MONOCYTIC ADAPTER / MONA / ADAPTER PROTEIN GRID
Mass: 6734.568 Da / Num. of mol.: 1 / Fragment: SH3C DOMAIN, RESIDUES 265-322 Source method: isolated from a genetically manipulated source Source: (gene. exp.) MUS MUSCULUS (house mouse) / Plasmid: PGEX-6P-1 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: O89100
Resolution: 1.5→34.71 Å / Cor.coef. Fo:Fc: 0.944 / Cor.coef. Fo:Fc free: 0.94 / SU B: 1.308 / SU ML: 0.051 / Cross valid method: THROUGHOUT / ESU R: 0.098 / ESU R Free: 0.089 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.221
485
4.8 %
RANDOM
Rwork
0.207
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obs
0.208
9573
99.3 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK