+Open data
-Basic information
Entry | Database: PDB / ID: 1uqv | ||||||
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Title | SAM domain from Ste50p | ||||||
Components | STE50 PROTEIN | ||||||
Keywords | SIGNALING PROTEIN / SAM / STERILE ALPHA MOTIF / HELICAL / PROTEIN-PROTEIN INTERACTION DOMAIN / GROWTH ARREST | ||||||
Function / homology | Function and homology information : / osmosensory signaling pathway via Sho1 osmosensor / signal transduction involved in filamentous growth / SAM domain binding / pheromone-dependent signal transduction involved in conjugation with cellular fusion / protein kinase regulator activity / regulation of cell cycle / cell cycle / cytoplasm Similarity search - Function | ||||||
Biological species | SACCHAROMYCES CEREVISIAE (brewer's yeast) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Grimshaw, S.J. / Mott, H.R. / Stott, K.M. / Nielsen, P.R. / Evetts, K.A. / Hopkins, L.J. / Nietlispach, D. / Owen, D. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2004 Title: Structure of the Sterile {Alpha} Motif (Sam) Domain of the Saccharomyces Cerevisiae Mitogen-Activated Protein Kinase Pathway-Modulating Protein Ste50 and Analysis of its Interaction with the Ste11 Sam Authors: Grimshaw, S.J. / Mott, H.R. / Stott, K.M. / Nielsen, P.R. / Evetts, K.A. / Hopkins, L.J. / Nietlispach, D. / Owen, D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1uqv.cif.gz | 652.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1uqv.ent.gz | 547.2 KB | Display | PDB format |
PDBx/mmJSON format | 1uqv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/uq/1uqv ftp://data.pdbj.org/pub/pdb/validation_reports/uq/1uqv | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 9897.152 Da / Num. of mol.: 1 / Fragment: SAM DOMAIN, RESIDUES 27-108 Source method: isolated from a genetically manipulated source Source: (gene. exp.) SACCHAROMYCES CEREVISIAE (brewer's yeast) Plasmid: PGEX-2T / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21 / References: UniProt: P25344 | ||
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Compound details | INVOLVED IN GROWTH ARREST DURING CONJUGATIOSequence details | THE FIRST THREE RESIDUES IN THE DBREF RECORDS SHOWN ORIGINATE FROM THE EXPRESSION SYSTEM, AND ARE ...THE FIRST THREE RESIDUES IN THE DBREF RECORDS SHOWN ORIGINATE FROM THE EXPRESSION | |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: THE STRUCURE WAS DETERMINED USING 15N-LABELLED AND 13C,15N-LABELLED PROTEINS |
-Sample preparation
Details | Contents: 16NM NA2HPO4, 4MM NAH2PO4, 50MM NACL, 5MM DTT, 10% D2O, 0.05% NAN3 |
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Sample conditions | Ionic strength: 260 mM / pH: 6.0 / Pressure: 1 atm / Temperature: 308 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: LOWEST ENERGY / Conformers calculated total number: 100 / Conformers submitted total number: 25 |